J Korean Med Sci.  2003 Aug;18(4):505-509. 10.3346/jkms.2003.18.4.505.

The Proteomics Approach to Find Biomarkers in Gastric Cancer

• Ryu JW
• Kim HJ
• Lee YS
• Myong NH
• Hwang CH
• Lee GS
• Yom HC

• KMID: 648901
• DOI: http://doi.org/10.3346/jkms.2003.18.4.505

Abstract

Gastric cancer is a very serious disease and is naturally resistant to many anticancer drugs. To reduce the mortality and improve the effectiveness of therapy, many studies have tried to find key biomarkers. Proteomic technologies are providing the tools needed to discover and identify disease-associating biomarkers. The proteomic study of gastric cancer establishes any specific events that lead to cancer, and it provides a direct way to define the true function of genes. Using two dimensional (2-D) electrophoresis of the stomach cancer tissue, we have gained about 1,500 spots in each gel, and 140 protein spots also were identified. Among the identified proteins, there were seven over-expressed proteins in stomach cancer tissue: NSP3, transgelin, prohibitin, heat shock protein (hsp) 27 and variant, protein disulfide isomerase A3, unnamed protein product and glucose regulated protein. There were also seven under-expressed proteins in stomach cancer: Apolipoprotein A-1, p20, nucleoside diphosphate isomerase A, alpha 1 antitrypsin, desmin, serum albumin and sero-transferrin.

Keyword

Proteomics; Stomach Neoplasms; Electrophoresis; Gel; Two-Dimensional

MeSH Terms

Aged
Carrier Proteins/biosynthesis
Cell Line, Tumor
Electrophoresis, Gel, Two-Dimensional
Female
Human
Male
Microfilament Proteins/biosynthesis
Middle Aged
Muscle Proteins/biosynthesis
Neoplasm Proteins/biosynthesis
Proteins/biosynthesis
*Proteome
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stomach Neoplasms/*metabolism
*Tumor Markers, Biological