J Korean Med Sci.  2003 Aug;18(4):467-472. 10.3346/jkms.2003.18.4.467.

Distinct Patterns of Cleavage and Translocation of Cell Cycle Control Proteins in CD95-induced and p53-induced apoptosis

• Park WS
• Jung KC
• Chung DH
• Nam WD
• Choi WJ
• Bae Y

• KMID: 648894
• DOI: http://doi.org/10.3346/jkms.2003.18.4.467

Abstract

Apoptotic cell death induced by p53 occurs at a late G1 cell cycle checkpoint termed the restriction(R)point, and it has been proposed that p53-induced apoptosis causes upregulation of CD95. However, as cells with defective in CD95 signaling pathway are still sensitive to p53-induced apoptosis, CD95 cannot be the sole factor resulting in apoptosis. In addition, unlike p53-induced apoptosis, the relationship between CD95-mediated apoptosis and the cell cycle is not clearly understood. It would there-fore be worth investigating whether CD95-mediated cell death is pertinent with p53-induced apoptosis in view of cell cycle related molecules. In this report, biochemical analysis showed that etoposide-induced apoptosis caused the induction and the nuclear translocation of effector molecules involved in G1 cell cycle checkpoint. However, there was no such translocation in the case of CD95-mediated death. Thus, although both types of apoptosis involved caspase activation, the cell cycle related proteins responded differently. This argues against the idea that p53-induced apoptosis occurs through the induction of CD95/CD95L expression.

Keyword

Etoposide; Cell Cycle; Nuclear Translocation; Caspase; DNA Damage; poptosis

MeSH Terms

Active Transport, Cell Nucleus
Antigens, CD95/*metabolism
*Apoptosis
Cell Cycle
Cell Nucleus/metabolism
Coculture
Dose-Response Relationship, Drug
Down-Regulation
Etoposide/pharmacology
Flow Cytometry
Human
Immunoblotting
Jurkat Cells
Nucleic Acid Synthesis Inhibitors/pharmacology
Protein Binding
Protein Transport
Protein p53/*metabolism
Signal Transduction
Up-Regulation