J Korean Med Sci.  1994 Aug;9(4):299-303. 10.3346/jkms.1994.9.4.299.

p53 protein overexpression and allele loss of p53 gene in gastric adenocarcinoma

• Choi SW
• Park WS
• Yang JM
• Kang CS
• Sun HS
• Kim BS
• Seo EJ
• Lee MJ
• Park CS

• KMID: 644812
• DOI: http://doi.org/10.3346/jkms.1994.9.4.299

Abstract

Gene alterations of p53 tumor suppressor gene such as point mutations, deletions or insertions occur in various human cancers. p53 protein overexpression was studied immunohistochemically in 80 gastric adenocarcinomas using an anti-human p53 antibody (Pab 1801) and the avidin-biotin-peroxidase technique. We have also analyzed allele loss of the human p53 gene in 54 cases of gastric adenocarcinoma using polymerase chain reaction and restriction fragment length polymorphism. p53 immunostaining was also demonstrated in 48 of 80 carcinomas (60%). Normal mucosa was always negative. No relation could be found between p53 immunostaining and the degree of differentiation. 21 of the 54 patients(39%) were informative for the p53 exon 4. In ten of these informative cases(47.6%), tumor DNAs showed allele loss when compared with nonmetastatic lymph node DNAs. Seven of the ten(70%) showed p53 immunoreactivity. These findings suggest that mutations of the p53 gene may play a role in the development of gastric adenocarcinoma and that allele loss of p53 frequently occurs in p53 immunoreactive gastric adenocarcinoma.

Keyword

gastric adenocarcinoma; p53 gene ; LOH; immunoreactivity

MeSH Terms

Adenocarcinoma/*genetics
*Alleles
Base Sequence
DNA, Neoplasm/*analysis
Gene Expression Regulation, Neoplastic/*genetics
Genes, p53/*genetics
Genetic Markers
Human
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Stomach Neoplasms/*genetics