Tissue Eng Regen Med.
2022 Dec;19(6):1295-1310. 10.1007/s13770-022-00490-0.
Mesenchymal Stem Cell Secreted-Extracellular Vesicles are Involved in Chondrocyte Production and Reduce Adipogenesis during Stem Cell Differentiation
- Affiliations
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- 1Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan
- 2Institute of Biopharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan
- 3Department of Medical Research, Far Eastern Memorial Hospital, New Taipei City 220216, Taiwan
- 4College of Management, Chung Hua University, Hsinchu 30012, Taiwan
- 5Gwo Xi Stem Cell Applied Technology Co., Ltd., Hsinchu 30261, Taiwan
- 6Department of Pharmacy, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan
- 7Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
- 8GTESTing Laboratory, Hsinchu 30261, Taiwan
- 9College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan
- 10Department of Orthopedics, Far Eastern Memorial Hospital, New Taipei City 22060, Taiwan
- 11Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 32003, Taiwan
- 12Department of Biochemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
- 13School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
- KMID: 2536459
- DOI: http://doi.org/10.1007/s13770-022-00490-0
Abstract
- BACKGROUND
Extracellular vesicles (EVs) are derived from internal cellular compartments, and have potential as a diagnostic and therapeutic tool in degenerative disease associated with aging. Mesenchymal stem cells (MSCs) have become a promising tool for functional EVs production. This study investigated the efficacy of EVs and its effect on differentiation capacity.
METHODS
The characteristics of MSCs were evaluated by flow cytometry and stem cell differentiation analysis, and a production mode of functional EVs was scaled from MSCs. The concentration and size of EVs were quantitated by Nanoparticle Tracking Analysis (NTA). Western blot analysis was used to assess the protein expression of exosomespecific markers. The effects of MSC-derived EVs were assessed by chondrogenic and adipogenic differentiation analyses and histological observation.
RESULTS
The range of the particle size of adipose-derived stem cells (ADSCs)- and Wharton’s jelly -MSCs-derived EVs were from 130 to 150 nm as measured by NTA, which showed positive expression of exosomal markers. The chondrogenic induction ability was weakened in the absence of EVs in vitro. Interestingly, after EV administration, type II collagen, a major component in the cartilage extracellular matrix, was upregulated compared to the EV-free condition. Moreover, EVs decreased the lipid accumulation rate during adipogenic induction.
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