Lab Anim Res.
2021 Sep;37(3):252-263. 10.1186/s42826-021-00097-x.
Establishment of particulate matterinduced lung injury model in mouse
- Affiliations
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- 1Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea
- 2Korea Conformity Laboratories, Gaetbeol-ro 145 beon-gil, Yeonsu-gu, Incheon 21999, South Korea
- 3Brain Korea 21 PLUS Project for Medical Science, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul 03722, South Korea
- 4College of Pharmacy, Gachon University, Incheon 21936, South Korea
- 5Univera Co., Ltd., Seoul 04782, South Korea
- KMID: 2521040
- DOI: http://doi.org/10.1186/s42826-021-00097-x
Abstract
- Background
Particulate matter (PM) is one of the principal causes of human respiratory disabilities resulting from air pollution. Animal models have been applied to discover preventive and therapeutic drugs for lung diseases caused by PM. However, the induced severity of lung injury in animal models using PM varies from study to study due to disparities in the preparation of PM, and the route and number of PM administrations. In this study, we established an in vivo model to evaluate PM-induced lung injury in mice.
Results
PM dispersion was prepared using SRM2975. Reactive oxygen species were increased in MLE 12 cells exposed to this PM dispersion. In vivo studies were conducted in the PM single challenge model, PM multiple challenge model, and PM challenge with ovalbumin-induced asthma using the PM dispersion. No histopathological changes were observed in lung tissues after a single injection of PM, whereas mild to moderate lung inflammation was obtained in the lungs of mice exposed to PM three times. However, fibrotic changes were barely seen, even though transmission electron microscopy (TEM) studies revealed the presence of PM particles in the alveolar macrophages and alveolar capillaries. In the OVA-PM model, peribronchial inflammation and mucous hypersecretion were more severe in the OVA+PM group than the OVA group. Serum IgE levels tended to increase in OVA+PM group than in OVA group.
Conclusions
In this study, we established a PM-induced lung injury model to examine the lung damage induced by PM. Based on our results, repeated exposures of PM are necessary to induce lung inflammation by PM alone. PM challenge, in the presence of underlying diseases such as asthma, can also be an appropriate model for studying the health effect of PM.
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