Osteoporos Sarcopenia.  2019 Dec;5(4):109-115. 10.1016/j.afos.2019.11.003.

Effects of teriparatide on bone in autochthonous transgenic model mice for diabetes mellitus (Akita mice)

• Ohuchi K
• Miyakoshi
• Kasukawa Y
• Segawa T
• Kinoshita
• Sato C
• Fujii M
• Shimada Y

• KMID: 2470596
• DOI: http://doi.org/10.1016/j.afos.2019.11.003

Abstract

OBJECTIVES
The purpose of this study is to evaluate the effects of teriparatide (TPTD) on bone mineral density (BMD), bone strength, and bone quality in Akita mouse models of diabetes mellitus.
METHODS
Twelve-week-old female Akita mice and control mice (C57/BL/6NCrSlc) were divided into 4 groups: control mice treated with vehicle (n = 7) or TPTD (n = 6); and Akita mice treated with vehicle (n = 6) or TPTD (n = 7). TPTD or vehicle was administered subcutaneously 3 times a week for 8 weeks. Blood glucose, serum sclerostin, total tibial BMD, femoral shaft bone strength, and bone quality using Fourier-transform infrared spectroscopy imaging were evaluated.
RESULTS
No significant differences in serum sclerostin levels were evident among these groups after 8 weeks of treatment. TPTD significantly increased BMD in control mice (+12.7%, P = 0.02) and Akita mice (+29.2%, P = 0.001) compared with vehicle. Maximum load and stiffness were significantly higher in Akita mice treated with TPTD than in Akita mice treated with vehicle (+56.6%, P = 0.03 and + 90.5%, P = 0.02, respectively). On Fourier-transform infrared spectroscopy imaging, the mineral/matrix ratio was significantly lower in Akita mice treated with vehicle than in control mice (−12.2%, P = 0.02), and TPTD treatment significantly increased the mineral/matrix ratio (P = 0.003).
CONCLUSIONS
TPTD thus improved BMD and bone strength in both control mice and Akita mice, with improvements in the mineral/matrix ratio among Akita mice.

Keyword

Diabetes mellitus; Akita mouse; Teriparatide; Bone mineral density; Bone strength; Bone quality

MeSH Terms

Animals
Blood Glucose
Bone Density
Diabetes Mellitus*
Female
Humans
Mice*
Spectrum Analysis
Teriparatide*
Blood Glucose
Teriparatide