Korean J Anat.  1999 Oct;32(5):727-733.

Positive Selection of Transgenic CD4 T Cells in the Restricted Peptide Environment

• Lee DS
• Lee WJ

Abstract

In order to investigate the role of H2-M molecule on class II molecule other than H2-Ab we made H2-M deficient H2-E transgenic mice and to see the peptide diversity on H2-E molecule in these mice, we contructed AND --> H2-M deficient H2-E transgenic bone marrow chimeras. The results were as followings. 1. The phenotype of thymus of H2-M deficient H2-E transgenic mice showed similar CD4 and CD8 single positive thymocyte amount as normal wild type B6 mice with CD4/CD8 ratio around 2. The percentage of double positive cells were about 85%. 2. In the periphery, H2-M deficient H2-E transgenic mice also showed similar amount of CD4 and CD8 cells as normal wild type B6 mice. The CD4/CD8 ratio was 1.5~2. 3. The development of transgenic CD4 cells from AND --> H2-M+/- H2-E transgenic bone marrow chimera was similar to wild type AND transgenic mice. 4. The development of transgenic CD4 cells from AND --> H2-M deficient H2-E transgenic bone marrow chimera was reduced compared to wild type AND transgenic mice or AND --> H2-M+/- H2-E transgenic bone marrow chimeras. But the amount of CD4 cells developed in these chimeras were significant and H2-E molecule could bind some self peptides in the absence of H2-M molecules and support the CD4 cell development in these chimeras.

Keyword

Mouse; CD4 T cell; Restricted peptide

MeSH Terms

Animals
Bone Marrow
Chimera
Mice
Mice, Transgenic
Peptides
Phenotype
T-Lymphocytes*
Thymocytes
Thymus Gland
Peptides