Environ Health Toxicol.
2016 ;31(1):e2016009. 10.5620/eht.e2016009.
Analysis of lysosomal membrane proteins exposed to melanin in HeLa cells
- Affiliations
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- 1Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea. jihomin@jbnu.ac.kr
- 2Department of Microbiology, Chungbuk National University, Cheongju, Korea.
- KMID: 2469665
- DOI: http://doi.org/10.5620/eht.e2016009
Abstract
OBJECTIVES
There have been developed to use targeting ability for antimicrobial, anticancerous, gene therapy and cosmetics through analysis of various membrane proteins isolated from cell organelles.
METHODS
It was examined about the lysosomal membrane protein extracted from lysosome isolated from HeLa cell treated by 100 ppm melanin for 24 hours in order to find associated with targeting ability to melanin using by 2-dimensional electrophoresis.
RESULTS
The result showed 14 up-regulated (1.5-fold) and 13 down-regulated (2.0-fold) spots in relation to melanin exposure.
CONCLUSIONS
It has been found that lysosomal membrane proteins are associated with melanin to decolorize and quantity through cellular activation of lysosome.
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