Yonsei Med J.  2020 Jan;61(1):20-29. 10.3349/ymj.2020.61.1.20.

Curcumin Inhibits the Proliferation, Migration, Invasion, and Apoptosis of Diffuse Large B-Cell Lymphoma Cell Line by Regulating MiR-21/VHL Axis

Affiliations
  • 1Department of Pathology, Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, Hubei, China.
  • 2Department of Gastrointestinal Surgery, Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, Hubei, China.
  • 3Cancer Center of Guangzhou Medical University, Guangzhou, China.
  • 4Administrative Department, Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, Hubei, China. hongyuzjhz@163.com

Abstract

PURPOSE
Curcumin exerts its anti-cancer effects, partly by targeting special microRNAs, in human cancers. MiR-21 is a key oncomir in carcinogenesis of multiple human cancers. Here, we aimed to further explore the mechanistic insight into the link between curcumin and miR-21 on diffuse large B-cell lymphoma (DLBCL).
MATERIALS AND METHODS
Quantitative real-time PCR assays were performed to assess the levels of miR-21 and Von Hippel-Lindau (VHL) mRNA. In situ hybridization assay was used for miR-21 expression visualization in lymphoma tissues. Western blot was used for determination of VHL protein, Ki-67, caspase-3, and cleaved caspase-3 levels. Dual-luciferase reporter assay and RNA immunoprecipitation assay were employed to confirm the direct target of miR-21. MTT assay, flow cytometric analysis, and transwell assay were used to evaluate cell proliferation, apoptosis, and migration and invasion capacities, respectively.
RESULTS
Curcumin repressed the proliferation, migration, and invasion abilities and promoted apoptosis in SU-DHL-8 cells. Curcumin inhibited miR-21 expression and curcumin exerted its anti-proliferation, anti-migration, anti-invasion, and pro-apoptosis effects by miR-21 in SU-DHL-8 cells. VHL was a direct target of miR-21. Moreover, curcumin exerted its regulatory effects on SU-DHL-8 cells by VHL.
CONCLUSION
Curcumin exerted its anti-proliferation, anti-migration, anti-invasion, and pro-apoptosis functions, at least partly, by repressing miR-21 and regulating VHL expression in DLBCL cell line. Our findings provided a possible molecular mechanism of curcumin-mediated anti-cancer effect.

Keyword

Diffuse large B-cell lymphoma (DLBCL); curcumin; miR-21; Von Hippel-Lindau (VHL)

MeSH Terms

Apoptosis*
B-Lymphocytes*
Blotting, Western
Carcinogenesis
Caspase 3
Cell Line*
Cell Proliferation
Curcumin*
Humans
Immunoprecipitation
In Situ Hybridization
Lymphoma
Lymphoma, B-Cell*
MicroRNAs
Real-Time Polymerase Chain Reaction
RNA
RNA, Messenger
Caspase 3
Curcumin
MicroRNAs
RNA
RNA, Messenger
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