Korean J Otorhinolaryngol-Head Neck Surg.  2019 Sep;62(9):507-514. 10.3342/kjorl-hns.2018.00920.

Inhibitory Effects of Protopanaxadiol on Lipopolysaccharide-Induced Reactive Oxygen Species Production and MUC5AC Expression in Human Airway Epithelial Cells

• Song YS
• Kim JH
• Na H
• Choi YS
• Song SY
• Kim YD
• Bae CH

• KMID: 2461002
• DOI: http://doi.org/10.3342/kjorl-hns.2018.00920

Abstract

BACKGROUND AND OBJECTIVES
MUC5AC is one of the major secretory mucin genes in the human airway epithelium. MUC5AC expression is increased by a variety of inflammatory mediators. Protopanaxadiol (PPD), one of the major active metabolites in ginseng, is known to have anti-inflammatory, antitumor and antioxidant properties. However, the effects of PPD on mucin secretion of airway epithelial cells still have not been reported. Therefore, the aim of this study is to investigate the effect of PPD on lipopolysaccharide (LPS)-induced MUC5AC expression in human airway epithelial cells. MATERIALS AND METHOD: In the mucin-producing human NCI-H292 airway epithelial cells, the effect of PPD on MUC5AC expression was investigated using reverse transcription-polymerase chain reaction and enzyme immunoassay after treated with LPS. N-acetylcysteine (NAC) as a reactive oxygen species (ROS) scavenger, and apocynin as a nicotinamide adenine dinucleotide phosphate oxidase inhibitor were used to compare the inhibitory effect of PPD on LPS-induced ROS production in human NCI-H292 cells.
RESULTS
LPS significantly increased MUC5AC mRNA expression and protein production. LPS also increased ROS production. PPD inhibited LPS-induced MUC5AC mRNA expression and protein production as well as ROS production. In addition, NAC and apocynin inhibited LPS-induced MUC5AC mRNA expression and protein production.
CONCLUSION
These results demonstrate that PPD inhibits LPS-induced MUC5AC expression via ROS in human airway epithelial cells and the inhibitory effect of PPD was similar to that of NAC and apocynin. These findings indicate that PPD may be a therapeutic agent for control of mucus secretion and oxidative stress in human airway epithelial cells.

Keyword

Human airway epithelial cells; MUC5AC; Protopanaxadiol; Reactive oxygen species

MeSH Terms

Acetylcysteine
Epithelial Cells*
Epithelium
Humans*
Immunoenzyme Techniques
Methods
Mucins
Mucus
NADP
Oxidative Stress
Oxidoreductases
Panax
Reactive Oxygen Species*
RNA, Messenger
Acetylcysteine
Mucins
NADP
Oxidoreductases
RNA, Messenger
Reactive Oxygen Species