Korean J Intern Med.  2019 Jan;34(1):178-183. 10.3904/kjim.2017.102.

Small RNA sequencing profiles of mir-181 and mir-221, the most relevant microRNAs in acute myeloid leukemia

• Lee YG
• Kim I
• Oh S
• Shin DY
• Koh Y
• Lee KW

• KMID: 2452501
• DOI: http://doi.org/10.3904/kjim.2017.102

Abstract

BACKGROUND/AIMS
To evaluate and select microRNAs relevant to acute myeloid leukemia (AML) pathogenesis, we analyzed differential microRNA expression by quantitative small RNA next-generation sequencing using duplicate marrow samples from individual AML patients.
METHODS
For this study, we obtained paired marrow samples at two different time points (initial diagnosis and first complete remission status) in patients with AML. Bone marrow microRNAs were profiled by next-generation small RNA sequencing. Quantification of microRNA expression was performed by counting aligned reads to microRNA genes.
RESULTS
Among 38 samples (32 paired samples from 16 AML patients and 6 normal marrow controls), 27 were eligible for sequencing. Small RNA sequencing showed that 12 microRNAs were selectively expressed at higher levels in AML patients than in normal controls. Among these 12 microRNAs, mir-181, mir-221, and mir-3154 were more highly expressed at initial AML diagnosis as compared to first complete remission. Significant correlations were found between higher expression levels of mir-221, mir-146, and mir-155 and higher marrow blast counts.
CONCLUSIONS
Our results demonstrate that mir-221 and mir-181 are selectively enriched in AML marrow and reflect disease activity. mir-3154 is a novel microRNA that is relevant to AML but needs further validation.

Keyword

MicroRNAs; Sequence analysis, RNA; Acute myeloid leukemia

MeSH Terms

Bone Marrow
Diagnosis
Humans
Leukemia, Myeloid, Acute*
MicroRNAs*
RNA*
Sequence Analysis, RNA*
MicroRNAs
RNA