J Cancer Prev.  2019 Mar;24(1):20-25. 10.15430/JCP.2019.24.1.20.

Rat Intestinal Acetic Acid and Butyric acid and Effects of Age, Sex, and High-fat Diet on the Intestinal Levels in Rats

Affiliations
  • 1Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea. nayoungkim49@empas.com
  • 2Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.
  • 3Department of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Korea.
  • 4Korea Institute of Science and Technology Natural Products Research Institute, Gangneung, Korea.

Abstract

BACKGROUND
High-fat diet is known to be implicated in the pathogenesis of various metabolic disorders related to an inflammatory response. The aim of this study was to investigate the influence of high-fat diet for intestinal acetic acid and butyric acid concentrations which are related to inflammation-associated colon cancer risk.
METHODS
Both male and female rats of 6, 31, 74 and 104-week of age were fed chow diet or high-fat diet for 8 weeks. Body weight and food intake were measured weekly during the feeding period. Intestinal acetic acid and butyric acid levels were measured by high-performance liquid chromatography from luminal contents of ileum and cecum.
RESULTS
Male rats showed greater weight change than female rats in every age. Calorie-adjusted food intake was also higher in male rats compared to female rats. Male rats showed similar intake of food in every age while 31-week old female rats showed increased intake, which was decreased at 74-week and 104-week of age. The ileal acetic acid concentration was increased in male rats fed high-fat diet, while female rats fed high-fat diet showed no significant change in the ileal acetic acid level. On the other hand, butyric acid almost disappeared in high-fat diet fed rats regardless of sex.
CONCLUSIONS
High-fat diet increases the intestinal acetic acid concentration while reducing the butyric acid concentration which may account for increased risk of inflammation-associated colon cancer.

Keyword

Acetic acid; Butyric acid; Rats; High-fat diets

MeSH Terms

Acetic Acid*
Animals
Body Weight
Butyric Acid*
Cecum
Chromatography, Liquid
Colonic Neoplasms
Diet
Diet, High-Fat*
Eating
Female
Hand
Humans
Ileum
Male
Phenobarbital
Rats*
Acetic Acid
Butyric Acid
Phenobarbital
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