Korean J Intern Med.  2019 Mar;34(2):390-400. 10.3904/kjim.2016.239.

Expression and prognostic significance of microRNAs in Korean patients with myelodysplastic syndrome

• Choi Y
• Hur EH
• Moon JH
• Goo BK
• Choi DR
• Lee JH

• KMID: 2441019
• DOI: http://doi.org/10.3904/kjim.2016.239

Abstract

BACKGROUND/AIMS
Various alterations of microRNA (miRNA) expression have been reported in myelodysplastic syndrome (MDS). We aimed to investigate the unique patterns and prognostic significance of miRNA expression in Korean patients with MDS.
METHODS
Bone marrow mononuclear cells were collected from eight healthy controls and 26 patients with MDS, and miRNAs were isolated and assessed via quantitative real-time polymerase chain reaction for selected miRNAs, including miR-21, miR-124a, miR-126, miR-146b-5p, miR-155, miR-182, miR-200c, miR-342-5p, miR-708, and Let-7a.
RESULTS
MiR-124a, miR-155, miR-182, miR-200c, miR-342-5p, and Let-7a were significantly underexpressed in patients with MDS, compared to healthy controls. MiR-21, miR-126, 146b-5p, and miR-155 transcript levels were significantly lower in international prognostic scoring system lower (low and intermediate-1) risk MDS than in higher (intermediate-2 and high) risk MDS. Higher expression levels of miR-126 and miR-155 correlated with significantly shorter overall survival and leukemia-free survival. Higher miR-124a expression also tended to be related to shorter survivals.
CONCLUSIONS
Although our study was limited by the relatively small number of patients included, we identified several miRNAs associated with pathogenesis, leukemic transformation, and prognosis in MDS.

Keyword

Myelodysplastic syndromes; MIRN126 microRNA, human; hsa-146b-5p; MIRN155 microRNA, human; MIRN200 microRNA, human

MeSH Terms

Bone Marrow
Humans
MicroRNAs*
Myelodysplastic Syndromes*
Prognosis
Real-Time Polymerase Chain Reaction
MicroRNAs