Korean J Vet Res.  2018 Dec;58(4):177-182. 10.14405/kjvr.2018.58.4.177.

Isolation and identification of canine adenovirus type 2 from a naturally infected dog in Korea

• Yang DK
• Kim HH
• Yoon SS
• Lee H
• Cho IS

• KMID: 2437692
• DOI: http://doi.org/10.14405/kjvr.2018.58.4.177

Abstract

Canine adenovirus type 2 (CAV-2) infection results in significant respiratory illness in dogs. Isolating and culturing CAV-2 allows for investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated a virus from a naturally infected dog in Gyeonggi-do, Korea. The virus was propagated in Madin-Darby canine kidney (MDCK) and Vero cells and showed a specific cytopathic morphology that appeared similar to a bunch of grapes. The virus was first confirmed as CAV-2 based on these cytopathic effects, an immunofluorescence assay, hemagglutination assay, and electron microscopy. The viral titer of the isolate designated APQA1601 reached 10(6.5) 50% tissue culture infections dose per mL in MDCK cells and exhibited no hemagglutination units with erythrocytes from guinea pig. The virus was also confirmed by polymerase chain reaction and next-generation sequencing. The APQA1601 strain had the highest similarity (~99.9%) with the Toronto A26/61 strain, which was isolated in Canada in 1976 when the nucleotide sequences of the full genome of the APQA1601 strain were compared with those of other CAV strains. Isolating CAV-2 will help elucidate the biological properties of CAV-2 circulating in Korean dogs.

Keyword

canine adenovirus type 2; dogs; virus isolaltion

MeSH Terms

Adenoviruses, Canine*
Animals
Base Sequence
Canada
Dogs*
Erythrocytes
Fluorescent Antibody Technique
Genome
Guinea Pigs
Gyeonggi-do
Hemagglutination
Kidney
Korea*
Madin Darby Canine Kidney Cells
Microscopy, Electron
Polymerase Chain Reaction
Vaccines
Vero Cells
Vitis
Vaccines