J Vet Sci.  2020 Jul;21(4):e63. 10.4142/jvs.2020.21.e63.

Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera

• Yang DK
• Kim HH
• Lee S
• Oh D
• Yoo JY
• Hyun BH

• KMID: 2504874
• DOI: http://doi.org/10.4142/jvs.2020.21.e63

Abstract

Background
Canine adenovirus type 2 (CAV-2) induces infectious laryngotracheitis in members of the family Canidae, including dogs. To date, no ELISA kits specific for CAV-2 antibody have been commercialized for dogs in Korea.
Objectives
We aimed to develop new indirect enzyme-linked immunosorbent assay (I-ELISA) to perform rapid, accurate serological surveys of CAV-2 in dog serum samples.
Methods
In total, 165 serum samples were collected from dogs residing in Chungbuk and Gyeongbuk provinces between 2016 and 2018. The Korean CAV-2, named the APQA1701-40P strain, was propagated in Madin–Darby canine kidney cells and purified in an anion-exchange chromatography column for use as an antigen for I-ELISA. The virus-neutralizing antibody titers of CAV-2 in the dog sera were measured by virus neutralization (VN) test.
Results
We compared the results obtained between the VN and new I-ELISA tests. The sensitivity, specificity, and accuracy of new I-ELISA were 98.6%, 86.4% and 97.0% compared with VN test, respectively. New I-ELISA was significantly correlated with VN (r = 0.91).
Conclusions
These results indicate that new I-ELISA is useful for sero-surveillance of CAV-2 in dog serum.

Keyword

Canine adenovirus type 2; antibody; ELISA