Ann Dermatol.  2012 May;24(2):181-188.

The Effect of Adipose-Derived Stem Cell-Cultured Media on Oxazolone Treated Atopic Dermatitis-Like Murine Model

Affiliations
  • 1Department of Dermatology, Yonsei University Wonju College of Medicine, Wonju, Korea. choieh@yonsei.ac.kr

Abstract

BACKGROUND
A stem cell is an undifferentiated cell that has the potential for self-renewal and differentiation. Adipose-derived stem cells (ADSCs) have advantages in accessibility and abundance compared to other kinds of stem cells and produce many growth factors and hormones.
OBJECTIVE
We investigated whether ADSC cultured media could be used as a therapy for atopic dermatitis.
METHODS
ADSC cultured media was topically applied twice daily for 5 days to oxazolone-treated atopic dermatitis-like hairless mice.
RESULTS
Topical application of ADSC cultured media improved the epidermal permeability barrier and keratinocyte differentiation, and restored the predominant Th2 phenotype when compared to vehicle. ADSC cultured media-treated epidermis also showed an increase in the expression of antimicrobial peptides cathelin-related antimicrobial peptide, mouse beta-defensein 3.
CONCLUSION
Topical ADSC cultured media could be useful in the treatment of atopic dermatitis.

Keyword

Adipose-derived stem cell; Atopic dermatitis; Oxazolone

MeSH Terms

Animals
Cathelicidins
Dermatitis, Atopic
Epidermis
Intercellular Signaling Peptides and Proteins
Keratinocytes
Mice
Oxazolone
Peptides
Permeability
Phenotype
Stem Cells
Cathelicidins
Intercellular Signaling Peptides and Proteins
Oxazolone
Peptides

Figure

  • Fig. 1 Methods of multiple oxazolone (Ox) challenges and topical administration of adipose-derived stem cell cultured media.

  • Fig. 2 (A) Basal TEWL (gm-2h-1) was checked after the fifth challenge of oxazolone (MM-pre, AM-pre, FM-pre, S-pre). The AM group and S group showed significant decreases in TEWL after treatment (C-post, MM-post, AM-post, FM-post and S-post). (B) SC hydration after treatment. AM, FM, and S groups showed significant improvement of SC hydration. Data are expressed as mean±standard deviation; n=6 in MM, AM, and FM group; n=4 in C group; n=3 in S group. TEWL: transepidermal water loss, SC: stratum corneum, C: control group, MM: minimal media applied group, AM: adipose-derived stem cell cultured media applied group, FM: fibroblast cultured media applied group, S: steroid applied group.

  • Fig. 3 Gross lesions of pre- and post-treatment with media in the atopic dermatitis model. The AM group and S group showed an improvement in skin lesions grossly. C: control group, MM: minimal media applied group, AM: adipose-derived stem cell cultured media applied group, FM: fibroblast cultured media applied group, S: steroid applied group.

  • Fig. 4 Th2 immunophenotype (CRTH2) and keratinocyte differentiation (filaggrin, involucrin, and loricrin) after treatment. Immunohistochemical staining was carried out with diaminobenzidine and primary antibodies as indicated. Merged images with propidium iodide secondary staining in 5 mm-thick frozen sections. C: control group, MM: minimal media applied group, AM: adipose-derived stem cell cultured media applied group, FM: fibroblast cultured media applied group, S: steroid applied group (Mag bars=2 mm).

  • Fig. 5 Topical application of adipose-derived stem cell cultured media enhanced the epidermal expression of antimicrobial peptides such as mBD3 and CRAMP. Immunohistochemical staining was carried out with diaminobenzidine and primary antibodies as indicated. mBD3: mouse beta-defensin 3, CRAMP: cathelin-related antimicrobial peptide, C: control group, MM: minimal media applied group, AM: adipose-derived stem cell cultured media applied group, FM: fibroblast cultured media applied group, S: steroid applied group (Mag bars=2 mm).

  • Fig. 6 Topical application of adipose-derived stem cell cultured media increased the protein and mRNA levels of mBD3 and CRAMP compared with other treatments in Western blots (A) and for mRNA of mBD3 and CRAMP compared with vehicle in real-time reverse transcriptase-polymerase chain reaction (B). This is compatible with immunohistochemical staining results. Data are expressed as mean±standard deviation; n=6 in MM, AM and FM group; n=4 in C group; n=3 in S group. CRAMP: cathelin-related antimicrobial peptide; mBD3: mouse beta-defensin 3, C: control group, MM: minimal media applied group, AM: adipose-derived stem cell cultured media applied group, FM: fibroblast cultured media applied group, S: steroid applied group.


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