Korean J Otorhinolaryngol-Head Neck Surg.  2019 Jan;62(1):28-35. 10.3342/kjorl-hns.2018.00164.

Effect of Resolvin D1 and E1 on Mucin Expression in Human Airway Epithelial Cells

• Kim HG
• Choi TY
• Bae CH
• Choi YS
• Na HG
• Song SY
• Kim YD

• KMID: 2433171
• DOI: http://doi.org/10.3342/kjorl-hns.2018.00164

Abstract

BACKGROUND AND OBJECTIVES
Mucin is an important component of mucus that performs the first line of defense against inhaled pathogens and particles, lubrication of organs, and protection of airway. It is hyper-secreted in inflammatory airway diseases and is associated with morbidity and mortality of the affected patients. Resolvin, an autacoid of a specific lipid structure, exhibits anti-inflammatory property against inflammatory airway diseases although its effects on mucin secretion by human airway epithelial cells have not yet been demonstrated. In this regard, we investigated the effects of Resolvin on lipopolysaccharide (LPS)-induced mucin expression in human airway epithelial cells. MATERIALS AND METHOD: In mucin-producing human NCI-H292 epithelial cells, the effects and brief signaling pathways of Resolvin D1 (RvD1) and Resolvin E1 (RvE1) on the LPS-induced MUC4, MUC5AC, and MUC5B expression were investigated using reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay and Western blot analysis.
RESULTS
RvD1 attenuated LPS-induced MUC4, MUC5AC, and MUC5B mRNA expression and protein production in human NCI-H292 cells while RvE1 did not. RvD1 significantly blocked LPS-induced activated phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) and p38 MAPK and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) while RvE1 did not.
CONCLUSION
These results suggest that RvD1 attenuates LPS-induced MUC4, MUC5AC, and MUC5B expressions via ERK1/2 MAPK, p38 MAPK, and NF-κB signaling pathways in airway epithelial cells. Therefore, RvD1 may modulate the control of mucus-hypersecretion in inflammatory airway diseases.

Keyword

Human airway epithelial cells; MUC4; MUC5AC; MUC5B; Resolvin

MeSH Terms

B-Lymphocytes
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Epithelial Cells*
Humans*
Lubrication
Methods
Mortality
Mucins*
Mucus
p38 Mitogen-Activated Protein Kinases
Phosphorylation
Phosphotransferases
Protein Kinases
RNA, Messenger
Mucins
Phosphotransferases
Protein Kinases
RNA, Messenger
p38 Mitogen-Activated Protein Kinases