Yonsei Med J.  2016 Nov;57(6):1461-1467. 10.3349/ymj.2016.57.6.1461.

Suppression of MicroRNA let-7a Expression by Agmatine Regulates Neural Stem Cell Differentiation

Affiliations
  • 1Department of Anatomy, Yonsei University College of Medicine, Seoul, Korea. jelee@yuhs.ac
  • 2Brain Korea 21 Plus Project for Medical Sciences and Brain Research Institute, Yonsei University College of Medicine, Seoul, Korea.

Abstract

PURPOSE
Neural stem cells (NSCs) effectively reverse some severe central nervous system (CNS) disorders, due to their ability to differentiate into neurons. Agmatine, a biogenic amine, has cellular protective effects and contributes to cellular proliferation and differentiation in the CNS. Recent studies have elucidated the function of microRNA let-7a (let-7a) as a regulator of cell differentiation with roles in regulating genes associated with CNS neurogenesis.
MATERIALS AND METHODS
This study aimed to investigate whether agmatine modulates the expression of crucial regulators of NSC differentiation including DCX, TLX, c-Myc, and ERK by controlling let-7a expression.
RESULTS
Our data suggest that high levels of let-7a promoted the expression of TLX and c-Myc, as well as repressed DCX and ERK expression. In addition, agmatine attenuated expression of TLX and increased expression of ERK by negatively regulating let-7a.
CONCLUSION
Our study therefore enhances the present understanding of the therapeutic potential of NSCs in CNS disorders.

Keyword

Neural stem cell (NSCs); microRNA let-7a (let-7a); agmatine; DCX; TLX; ERK

MeSH Terms

Agmatine/*pharmacology
Cell Differentiation/*drug effects
Cell Proliferation
Humans
MicroRNAs/*metabolism
Neural Stem Cells/cytology/*drug effects
Neurogenesis/*drug effects
Neurons/cytology/drug effects/metabolism
MicroRNAs
Agmatine
Full Text Links
  • YMJ
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error