Korean J Dermatol.  1997 Dec;35(6):1151-1158.

Tyrosine Protein Kinase (trk) Receptors Expression in Malignant Melanoma Cells

• Lee JY
• Park YL
• Whang KU

Abstract

BACKGROUND: Nerve growth factor(NGF), brain derived neurotrophic factor(BDNF), neurotropin 3(NT-3) and neurotropir-4/5 are neurotrophic factors necessary for the development and maintenance of specific neurors. The tyrosine protein kinase(trk) receptors exhibit specificity for differ ent neurotrophins. NGF is the cognate ligand for the trk A receptor, BDNF binds to trk B receptor and NT-3 binds to irk A, trk B and trk C receptors, Since melanoma cells are devived from neural ectoderm, growth factors which affect neuronal tissue may have a role in melanoma biology.
OBJECTIVE
The purpose of this study is to demonstrate the presence of trk receptors in rnelanoma cells and observe th effect of K-252a on these melanoma cells growth and differentiation.
METHODS
After K252a over a range of 0-200nM was added into their cell lines, we exam ined cell viability of SK 28 and SK 30 cells. We performed this to examine the expression of the trk by flow cytometry and immunoblotting.
RESULTS
1. The incubation of SK 28 cells and SK 30 cells with K 252a resulted in a dose dependent inhibition of cell proliferation. 2. In the flowcytometry, SK 28 cells and SK 30 cells showed a high expression of trk A and trk B, not trk C. 3. Using immunoblottiiig, trk in SK 28 cells and SK 30 cells was not expressed.
CONCLUSIONS
These results indicate that the identification of tyrosine protein kinase reeeptors and their inhibitor which affect differentiation and growth of a melanoma may provide an additional therapeutic option for treatment of melanoma.

Keyword

Neutrophic factors; Melanoma cells; Tyrosine protein kinase(trk) receptors

MeSH Terms

Biology
Brain
Cell Line
Cell Proliferation
Cell Survival
Ectoderm
Flow Cytometry
Immunoblotting
Intercellular Signaling Peptides and Proteins
Melanoma*
Nerve Growth Factor
Nerve Growth Factors
Neurons
Protein-Tyrosine Kinases*
Receptor, trkB
Sensitivity and Specificity
Tyrosine*
Intercellular Signaling Peptides and Proteins
Nerve Growth Factor
Nerve Growth Factors
Protein-Tyrosine Kinases
Receptor, trkB
Tyrosine