The effect of 1,25-dihydroxyvitamin D3 on the viability of periodontal ligament cells and the experimental tooth movement in rats

Kim, Sung Woo; Park, Dong Kwon; Kim, Sang Cheol

Abstract

. Vitamin D is known to exert its action by activating DNA and RBA within target cells to produce proteins and enzymes that can be used in bone resorption process. Particularly, the active form of vitmain D, 1,25-dihydroxycholecalciferol [1,25-(OH)2D3], is considered to be one of the most potent stimulators of osteoclatic acitivity in vitro. The purpose of this study was to evaluate the effect of1,25-Dihydroxyvitamin D3 on the avtivity of periodotal ligament cells and, the experimental tooth movement. Human periodontal ligament cells were collected from the first premolar tooth extracted for the orthodontic treatment, and were incubated in the environment of 37degreesC, 5% CO2 and 95% humidity. Microtitration{(MIT) assay was done at 10, 25, 50 and 100ng/ml of 1,25-Dihydroxyvitamin D3. 21 Sprague-Daft rats were divided into a control gmup(3), and experimental groups(18) where 100g of force from helical spring was applied across the maxillary incisors 1,25-Dihydroxyvitamin D3 was injected into periodontal ligament at the mesial or distal surface of maxillary incisors so that we can compare the control side and the experimental side. Expreimental groups were sac rifled at 12, 24, 36, 48, 72hours and 7 days after force application, respectively. And the obtained tissues were evaluated histologically. The observed results were as follows. 1. The activity of periodontal ligament cells in l0ng/ml or 25ng/ml of 1,25-Dihydroxyvitamin D31,25-Dihydroxyvitamin D3 was not significantly different to the control at the cultivation of 1, 2 and 3 days. 2. The activity of periodontal ligament cells was significantly increased at 3 days in 50 ng/ml of 1,25-Dihydroxyvitamin D3 and 2, 3 days in 100g/ml of 1,25-Dihydroxyvitamin D3. 3. Up to 7 days after force application, there was no difference in osteoblastic activity, tearing of periodontal ligament and proliferation of capillary at tension side between 1,25-Dihydroxyvitamin D3 injection side and the control side. 4. The osteoclastic activity and the resorption of alveolar bone was greater in 1,25-Dihydroxyvitamin D3 injection side than the control side at 36 hours after force application.