The effect of Vitamin D(3) and TGF-beta on the viability of human periodontal ligament cells

Song, Hyun Sup; Kim, Sang Cheol

Abstract

TGF-beta is a polypeptide with multiple physiological functions in regulation of cell-to-cell interaction and in growth and development. The active form of vitmain D3, 1,25-dihydroxycholecalciferol [1.25-(OH)2D3], is one of the most potent stimulators of osteoclastic acitivity. The purpose of this study was to evaluate the effect of Vitamin D3 and/or TGF-beta on the periodontal ligament(PDL) cells. Human PDL cells were prepared from the first premolars extracted for the orthodontic treatment and were incubated in the environment of 37degrees C, 5% CO2 and 95% humidity. 10, 50 or 100ng/ml of 1,25-(OH)2D3. and 0.1, 1, 5 or 10ng/ml of TGF-1beta were administered to the culture wells, separately or in combination. And the viability of PDL cells was evaluated by MTT assay. The obtained results were as follows. 1. The viability of PDL cells in 10ng/ml of vitamin D3 was not significantly differenent from that of the control group at 1, 2 and 3-day of cultivation, but it was significantly increased in 50ng/ml of Vitamin D3 at 3-day and in IOOng/ml of Vitamin D3 at 2 and 3-day. 2. The viability of PDL cells in 0.lng/ml of TGF-beta was not significantly differenent from that of the control group at 1, 2 and 3-day of cultivation, but it was significantly increased in 1 and 5ng/ml of TGF-beta at 3-day of cultivation, and in l0ng/mi of TGF-beta at 2 and 3-day of cultivation. 3. In case of admixture of lng/ml of TGF-beta and the various concentrations of vitamin D3, the viability of PDL cells was significantly increased in the admixture of 1OOng/ml of vitamin D3 at 3-day of cultivation. 4. In case of admixture of 5ng/ml of TGF-beta and the various concentrations of vitamin D3, the viability of PDL cells began to be increased from 2-day of cultivation in the admixture of 10 50 and lOOng/ml of vitamin D3, but it was not maintained at 3-day in the admixture of lOng/m of vitamin D3. 5. In case of admixture of lOng/ml of TGF-beta and the various concentrations of vitamin D3, the viability of PDL cells was significantly increased in the admixture of 5Ong/mi of vitamin D3 at 2 and 3-day of cultivation, and in the admixture of lOOng/mi at 1, 2 and 3-day.