J Korean Acad Periodontol.
2002 Sep;32(3):457-473. 10.5051/jkape.2002.32.3.457.
The Effect of TGF-beta1 on Cellular Activity of Periodontal Ligament Cells activated by PDGF-BB
- Affiliations
-
- 1Department of Periodontology, College of Dentistry Kyungpook National University, Korea.
- KMID: 2191775
- DOI: http://doi.org/10.5051/jkape.2002.32.3.457
Abstract
- The purposes of this study is to evaluate the combination effects of TGF-beta1 and PDGF-BB on the periodontal ligament cells to use as a regeneration promoting agent of periodontal tissue. Human periodontal ligament cells were prepared from the first premolar tooth extracted for the orthodontic treatment and were cultured in DMEM/10% FBS at the 37degrees C, 5% CO2 incubator. Authors measured the DNA synthesis, total protein, collagen and noncollagenous protein synthesis according to the concentration of TGF-beta1, (1, 5ng/ml) and PDGF-BB (1, 10 ng/ml) in combination. To explore further this delayed effect of TGF-beta1, we preincubated human periodontal ligament cells with TGF-beta1 for 4 or 24 hours before PDGF-BB stimulation. The results were as follows: The DNA synthetic activity was increased dose dependently by TGF-beta1, PDGF-BB. The combination of TGF-beta1 and PDGF-BB consistently enhanced the DNA synthetic activity to PDGF-BB alone. The ability of TGF-beta1 to enhance DNA synthetic activity in PDGF-BB treated periodontal ligament cells was dose dependent. The maximum mitogenic effect was at the 5ng/ml of TGF-beta1 and 10ng/ml of PDGF-BB. Preincubation of cells with TGF-beta1 resulted in significantly greater response to PDGF-BB at all TGF-beta1 concentration studied, and may be useful for clinical application in periodontal regenerative procedures. The total protein, collagen and noncollagen synthesis was increased dose pendently by TGF-beta1, PDGF-BB. The % of collagen was slightly decresed according to the concentration of TGF-beta1, PDGF-BB. The effect of TGF-beta1, PDGF-BB were not specific for collagen synthesis since it also increased noncollagenous protein synthesis. This study demonstrates that PDGF-BB is major mitogens for human periodontal ligament cells in vitro, and supports a role for TGF-beta1 as a regulation of the mitogenic and total protein formation to PDGF-BB in these cells.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- The effects of PDGF and LPS on the viabillty of human periodontal ligament cells
- A study on the expression of glycosaminoglycans in the experimental tooth movement of rat and in cultured periodontal ligament cells
- The biologic effects of safflower(Carthamus tinctorius Linne') extract and Dipsasi Radix extract on periodontal ligament cells and osteoblastic cells
- The Metabolic and Morphological Effects of Transforming Growth Factor (TGF)-beta1 in Bovine Retinal Pigment Epithelial Cells
- Increased Growth Factors in Children with Mycoplasma pneumoniae Pneumonia
