A study on the expression of glycosaminoglycans in the experimental tooth movement of rat and in cultured periodontal ligament cells

Lee, Kyung Hwan; Lee, Jong Jin; Kang, Kyung hwa; Kim, Eun Cheol; Kim, Sang Cheol

Abstract

The purpose of this study was to evaluate 1) in vivo, the expression of chondroitin 4-sulfate (CH-4S), a structural element of glycosaminoglycans(GAGs), in periodontal tissue during the experimental movement of rat incisors, by labelled streptavidine biotin immunohistochemical staining for CH-4S, 2) In vitro, the expression of CH-4S in cultured human periodontal ligament(PDL) cells supplemented with 10ng/ml of TGF-beta1, 20ng/ml of PDGF-BB, 1ng/ml TNF-alpha, or 1microgram/ml LPS by western blot analysis. The results of this study were as follows : 1. The expression of CH-4S was stronger in pulp, PDL, osteoblasts, osteoclasts and osteocytes in experimental group than in control group, but was rare in dentin, and cementum of experimental groups, regardless of the duration of force application, which was not different from that of control group. 2. In experimental group, the expression of CH-4S in pulp began to increase at 1 day after force application and got to the highest degree at 7 days. After 14 days, the expression in CH-4S immunoreactivity was decreased, and became similar to that of control group at 28 days. 3. The expression of CH-4S in PDL was noted in adjacent to alveolar bone. PDL showed higher intensity of immunolabelling after 1 day of orthodontic tooth movement. And the expression was more stronger in the tension side than that of pressure side of PDL at 1 day, but more stronger in the pressure side than that of tension side of PDL at 4 days. After 7 days, a decrease in CH-4S expression was observed. 4. The expression of CH-4S in alveolar bone got to the highest degree at 4 days, and At 7 days, a decrease in CH-4S expression was observed. 5. PDGF-BB notably raised the expression of CH-4S in the PDL cells at 3 days of cultivation 6. The expression of CH-4S of PDL cells was decreased with the application of TNF-alpha at 1 day. 7. Admixture of TGF-beta1 and PDGF-BB got more expression of CH-4S in PDL as compared to only TGF-beta1 or PDGF-BB. A similar decrease of the expression of CH-4S was observed in the case of application of LPS or TNF-alpha.