Psychiatry Investig.  2018 Oct;15(10):1000-1006. 10.30773/pi.2018.08.21.2.

Effects of 5HT1A Activation on Depression Profile Following 5-HT Depletion in Rats Lacking Social Attachment Since Weanling

• Chang KJ
• Chen YJ
• Chung JY
• Lin CC
• Liu YP

• KMID: 2425512
• DOI: http://doi.org/10.30773/pi.2018.08.21.2

Abstract

OBJECTIVE
Post weanling isolation-reared (IR) rats are featured with depressive phenotype, yet its mechanism is not clearly defined particularly in terms of the involvement of central 5-HT1A receptors. The present study aims to examine the effects of 5HT1A activation on forced swim test (FST) in IR rats following 5-HT depletion.
METHODS
Social control (SOC) and IR rats received an intracerebraoventricular (ICV) injection of 5-HT depletion agent, 5,7-DHT. 14 days after the surgery, rats were assessed their performance in FST with or without the challenge with a 5-HT1A agonist, 8-OH-DPAT. Rats were then sacrificed for analyzing their 5-HT tissue levels and the expressions of their 5-HA1A receptors in prefrontal cortex (PFC), hippocampus (HPX), and amygdala (AMY).
RESULTS
5,7-DHT decreased the tissue concentration of 5-HT in both IR and SOC rats. IR rats were more immobile and less sensitive to the lesion-induced immobility, however this effect was reversed by acute challenge of 8-OH-DPAT. 5,7-DHT lesion increased the expression of PFC 5-HT1A receptors.
CONCLUSION
The integrity of central 5-HT system is developmentally crucial for the 5-HT1A-relevant depression profile in rats of social isolation.

Keyword

5-HT1A receptors; Depression; Isolation-rearing; Serotonin

MeSH Terms

8-Hydroxy-2-(di-n-propylamino)tetralin
Amygdala
Animals
Depression*
Hippocampus
Phenotype
Prefrontal Cortex
Rats*
Receptor, Serotonin, 5-HT1A
Serotonin 5-HT1 Receptor Agonists
Serotonin*
Social Control, Formal
Social Isolation
8-Hydroxy-2-(di-n-propylamino)tetralin
Receptor, Serotonin, 5-HT1A
Serotonin
Serotonin 5-HT1 Receptor Agonists