Ann Dermatol.  2018 Jun;30(3):322-330. 10.5021/ad.2018.30.3.322.

Investigation of Immune-Regulatory Effects of Mageumsan Hot Spring via Protein Microarray In Vitro

Affiliations
  • 1Department of Dermatology, Uijeongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Uijeongbu, Korea. jwkim52@catholic.ac.kr

Abstract

BACKGROUND
Empirical evidences for efficacy of hot spring (HS) water in inflammatory skin disorders have not been substantiated with sufficient, immunological "hard evidence". Mageumsan HS water, characterized by its weakly-alkaline properties and low total dissolved solids content, has been known to alleviate various immune-inflammatory skin diseases, including atopic dermatitis (AD).
OBJECTIVE
The trial attempted to quantitatively analyze in vitro expression levels of chemical mediators in cutaneous inflammation from HaCaT cell line treated with Mageumsan HS, and suggest the likely mode of action through which it exerts the apparent anti-inflammatory effects in AD.
METHODS
Using membrane-based human antibody array kit, customized to include 30 different, keratinocyte-derived mediator proteins, their expression levels (including interleukin [IL]-1, IL-6, IL-8, thymic stromal lymphopoietin, thymus and activation-regulated chemokine, and granulocyte macrophage colony-stimulating factor) were assessed in vitro. Selected key proteins were further quantified with enzyme-linked immunosorbent assay.
RESULTS
There was a clear pattern of overall suppression of the mediators, especially those noted for their pro-inflammatory role in AD (monocyte chemoattractant protein [MCP]-1, regulated on activation, normal T cell expressed and secreted, cutaneous T-cell-attracting chemokine, Eotaxin, and macrophage inflammatory protein-1α, etc.). Also, reduced expression of involucrin and cytokeratin 1 was also reduced in the HS-treated group.
CONCLUSION
The present study has shown that Mageumsan HS water may exert its effects on inflammatory skin disorders through regulation of proinflammatory cytokines. These evidences are to be supported with further future investigations to elucidate immunological mechanism behind these beneficial effects of HS water in the chronically inflamed skin of AD.

Keyword

Atopic dermatitis; Hot spring water; Immune-regulation; Keratinocyte-derived cytokines/chemokines; Protein array analysis

MeSH Terms

Cell Line
Chemokine CCL17
Chemokine CCL27
Cytokines
Dermatitis, Atopic
Enzyme-Linked Immunosorbent Assay
Granulocytes
Hot Springs*
Humans
In Vitro Techniques*
Inflammation
Interleukin-6
Interleukin-8
Interleukins
Keratins
Macrophages
Protein Array Analysis*
Skin
Skin Diseases
Water
Chemokine CCL17
Chemokine CCL27
Cytokines
Interleukin-6
Interleukin-8
Interleukins
Keratins
Water
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