J Vet Sci.  2018 Jul;19(4):500-504. 10.4142/jvs.2018.19.4.500.

Oxidative stress and hepatic injury induced in mice fed a Sarcocystis hirsuta cyst extract

Affiliations
  • 1Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol 98613635856, Iran. nabavir796@gmail.com
  • 2Department of Basic Veterinary Science, Faculty of Veterinary Medicine, University of Zabol, Zabol 98613635856, Iran.
  • 3Department of Biology, School of Basic Sciences, University of Zabol, Zabol 98613635856, Iran.

Abstract

We studied the toxic effects of a Sarcocystis hirsuta cyst extract fed to mice. Degenerative changes were found in mice gavage-fed fresh, frozen, and heat-treated S. hirsuta cyst extract. There were increases in the levels of serum aspartate aminotransferase and alanine aminotransferase as well as hepatic and brain malondialdehyde (MDA) levels along with concomitant decreases in catalase (CAT) and superoxide dismutase (SOD) activities of mice receiving fresh and frozen S. hirsuta extracts. Gavage feeding of heat-treated S. hirsuta cyst extract had no effects on liver enzymes or brain MDA content, but the liver MDA level did increase. Mice in the heat-treated cyst group showed reduced CAT and SOD activities as well as increased hepatic MDA levels compared to those in the control group. These results indicate that an extract of S. hirsuta cyst can induce oxidative stress and hepatic injury, even after heat treatment.

Keyword

Sarcocystis hirsuta; alanine aminotransferase; aspartate aminotransferases; malondialdehyde; mice

MeSH Terms

Alanine Transaminase
Animals
Aspartate Aminotransferases
Brain
Catalase
Cats
Hot Temperature
Liver
Malondialdehyde
Mice*
Oxidative Stress*
Sarcocystis*
Superoxide Dismutase
Alanine Transaminase
Aspartate Aminotransferases
Catalase
Malondialdehyde
Superoxide Dismutase

Figure

  • Fig. 1 Serum superoxide dismutase (SOD; A) and catalase (CAT; B) activity levels in mice treated with Sarcocystis hirsuta extracts (mean ± SD, n = 8). *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the control group.

  • Fig. 2 Malondialdehyde (MDA) levels in liver (A) and brain (B) of mice treated with Sarcocystis hirsuta extracts (mean ± SD, n = 8). *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the control group.

  • Fig. 3 Serum levels of aspartate aminotransferase (AST; A) and alanine aminotransferase (ALT; B) in mice treated with Sarcocystis hirsuta extracts (mean ± SD, n = 8). *p < 0.05 and **p < 0.01 compared to the control group.

  • Fig. 4 Photomicrographs of liver sections of the experimental groups. (A) Normal histological appearance of liver tissue of a control mouse. (B) Non-infected extract treated mouse. (C) Fresh extract treated mouse, sinusoidal distention. (D) Liver tissue of a mouse receiving frozen extract, sinusoidal distention. (E) Liver tissue of a mouse received heated extract. H&E stain. 40× (A–E). White arrows, sinusoidal distention; black arrows, the pointer of the microscope.


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