Investig Clin Urol.  2018 Jul;59(4):252-256. 10.4111/icu.2018.59.4.252.

Measuring urinary 8-hydroxy-2′-deoxyguanosine and malondialdehyde levels in women with overactive bladder

• Dokumacioglu E
• Demiray O
• Dokumacioglu A
• Sahin A
• Sen TM
• Cankaya S

• KMID: 2414758
• DOI: http://doi.org/10.4111/icu.2018.59.4.252

Abstract

PURPOSE
In this study, we aimed to explain the role of oxidative stress in women with overactive bladder (OAB) by investigating the levels of 8-hydroxy-2"²-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage, and malondialdehyde (MDA), an indicator of lipid peroxidation.
MATERIALS AND METHODS
A total of 90 women were included in the study: 45 female patients diagnosed with OAB at Hopa State Hospital Urology Polyclinic and 45 healthy women without any metabolic or neurologic disease. Levels of MDA and 8-OHdG were measured in 24-hour urine samples for all subjects.
RESULTS
Urinary levels of MDA and 8-OHdG were significantly higher in the OAB group than in the control group (p < 0.001). A significant positive correlation (p<0.001) was found between the measurements of 8-OHdG and MDA.
CONCLUSIONS
Oxidative stress may be important in the pathophysiology of OAB, because levels of 8-OHdG and MDA are increased. Increased levels of 8-OHdG may be due to damaged nuclear and mitochondrial DNA as a result of oxidative attacks caused by free radicals. Nevertheless, further randomized and prospective studies with larger patient populations are needed.

Keyword

Lipid peroxidation; Oxidative stress; Urinary bladder, overactive; 8OHdG

MeSH Terms

DNA Damage
DNA, Mitochondrial
Female
Free Radicals
Hospitals, State
Humans
Lipid Peroxidation
Malondialdehyde*
Oxidative Stress
Prospective Studies
Urinary Bladder, Overactive*
Urology
DNA, Mitochondrial
Free Radicals
Malondialdehyde

Figure

• 

  Fig. 1 Urinary MDA levels of the patient and control groups. MDA, malondialdehyde; OAB, overactive bladder. a:Significantly different from control group (p<0.001).

• 

  Fig. 2 Urinary 8-OHdG levels of the patient and control groups. 8-OHdG, 8-hydroxy-2′-deoxyguanosine; OAB, overactive bladder. a:Significantly different from the control group (p<0.001).

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