J Korean Acad Oral Health.  2018 Mar;42(1):9-15. 10.11149/jkaoh.2018.42.1.9.

Inhibitory effect of 18β-glycyrrhetinic acid on the biofilm formation of Streptococcus mutans

Affiliations
  • 1LG Household & Health Care Ltd., Daejeon, Korea. shleek@lgcare.com

Abstract


OBJECTIVES
The present study aimed at investigating the potential of using 18β-glycyrrhetinic acid against the cariogenic characteristics of Streptococcus mutans UA159.
METHODS
The effects of 18β-glycyrrhetinic acid on biofilm formation and acid production were evaluated; the latter are indicators of cariogenicity of S. mutans. Biofilm architecture was also analyzed by scanning electron microscopy (SEM), and changes in gene expression related to biofilm formation were studied by quantitative RT-PCR.
RESULTS
Treatment with 18β-glycyrrhetinic acid at a concentration of 20 µg/ml inhibited biofilm formation by 95% in the absence of sucrose and 60% in its presence, reduced acid production by 88.8%, and significantly suppressed the gene expression of comDE, gbpB, gtfC and vicR, which are thought to be involved in the virulence of S. mutans.
CONCLUSIONS
These results suggest that 18β-glycyrrhetinic acid could be used as a complementary or alternative agent for preventing dental caries by interfering with the virulence properties of S. mutans without affecting the viability of the bacterial population.

Keyword

18β-glycyrrhetinic acid; Biofilm inhibition; S. mutans; Dental caries

MeSH Terms

Biofilms*
Dental Caries
Gene Expression
Microscopy, Electron, Scanning
Streptococcus mutans*
Streptococcus*
Sucrose
Virulence
Sucrose
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