J Korean Acad Oral Health.  2016 Jun;40(2):79-84. 10.11149/jkaoh.2016.40.2.79.

Gingival absorption of α-tocopherol acetate and 18β-glycyrrhetinic acid : in vitro evaluation in reconstructed gingival tissue

• Kim YS
• Kim JA
• You A
• Cho H
• Shin JY
• Lee S

• KMID: 2328811
• DOI: http://doi.org/10.11149/jkaoh.2016.40.2.79

Abstract

OBJECTIVES
To assess the absorption of α-tocopherol acetate and 18β-glycyrrhetinic acid, which are used as active ingredients in toothpaste, into a reconstructed gingival tissue.
METHODS
EpiGingival™ tissues were treated with a 25% slurry of toothpaste containing 2% α-tocopherol acetate and 0.3% 18β-glycyrrhetinic acid, for 2 minutes. The treatment was repeated up to 6 times, with 1 hour intervals. After completion of all treatments, the active ingredients in the tissue extracts and receiver solutions were measured by high performance liquid chromatography.
RESULTS
Although α-tocopherol acetate was not detected, α-tocopherol was detected in the tissue extracts, indicating that α-tocopherol acetate was bioconverted to α-tocopherol after absorption. We could detect 18β-glycyrrhetinic acid both in the tissue extracts and in the receiver solutions, with a positive correlation to the number of treatments.
CONCLUSIONS
We found that our toothpaste effectively delivered α-tocopherol acetate and 18β-glycyrrhetinic acid to a reconstructed gingival tissue in vitro.

Keyword

Absorption; α-Tocopherol acetate; 18β-Glycyrrhetinic acid; Periodontal disease; Toothpaste

MeSH Terms

Absorption*
Chromatography, Liquid
In Vitro Techniques*
Periodontal Diseases
Tissue Extracts
Toothpastes
Tissue Extracts
Toothpastes

Figure

• 

  Fig. 1. HPLC analysis. (A) α-tocopherol acetate (B) α-tocopherol (C)18β-glycyrrhetinic acid and (D) dipotassium glycyrrhizate. The top figures represent standards and the bottom ones represent the test samples. Arrows are the peaks of the target materials.

• 

  Fig. 2. Absorption and metabolism of α-tocopherol acetate. Toothpaste slurries were treated onto the EpiGingivalTM surfaces for 2 minutes. Treatments were repeated 2, 4, or 6 times. After all treatment, α-tocopherol (α-Toc) was measured by HPLC.

• 

  Fig. 3. Absorption of 18β-glycyrrhetinic acid. After repeated treatment of 25% toothpaste slurries, 18β-glycyrrhetinic acid (GA) was measured in the tissue extracts (A) and the assay media (B).

• 

  Fig. 4. Comparision of 18β-glycyrrhetinic acid and dipotassium glycyrrhizate. After repeated treatment of 25% toothpaste slurries 6 times, 18β-glycyrrhetinic acid (GA) and dipotassium glycyrrhizate (DPG) was measured in the tissue extracts (A) and the assay media (B). *P<0.05.

Reference

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