Lab Anim Res.  2017 Jun;33(2):132-139. 10.5625/lar.2017.33.2.132.

Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains

• Kim HR
• Choi JY
• Kim KS
• Jung YS
• Cho JY
• Hwang DY
• Song HK

• KMID: 2407427
• DOI: http://doi.org/10.5625/lar.2017.33.2.132

Abstract

Adaptive immunity is a type of immune response mediated by T and B cells, and is important response for immune response amplification and memory. In this study, the adaptive immunologic properties of C57BL/6NKorl substrain were compared with those of two other C57BL/6N substrains. There were no significant differences between the C57BL/6NKorl and the two other C57BL/6N substrains in the histological structures of the thymus and spleen, which are immunologic organs containing T cell and B cells. In addition, flow cytometric analysis did not reveal any significant differences in the distribution of T and B cell populations of the three substrains. To evaluate cell-mediated immunity of T cells in the three different substrains, we treated isolated T cells from spleen with Con A. The T cells of C57BL/6NKorl showed Con A-dependent proliferation of T cells at lower cell number than those in T cells from the other two C57BL/6N substrains. B cell-mediated humoral immune responses were not significant different among the three substrains. Thus, the results of this study provide evidence that C57BL/6NKorl mice are similar to those two other C57BL/6N substrains in humoral immunity, but C57BL/6NKorl has stronger response in cell mediated immunity.

Keyword

C57BL/6N; C57BL/6NKorl; T cell; B cell; cell-mediated immunity; humoral immunity

MeSH Terms

Adaptive Immunity
Animals
B-Lymphocytes
Cell Count
Immunity, Cellular*
Immunity, Humoral
Memory
Mice*
Spleen
T-Lymphocytes
Thymus Gland

Figure

• 

  Figure 1 Histological difference of thymus structure among C57BL/6NKorl, C57BL/6NA, and C57BL/6NB mice. Thymus tissues were stained with H&E. Upper line displayed at 100× magnification. Bottom line was viewed at 40× magnification. Arrow, cortex; arrow head, medulla.

• 

  Figure 2 Histological difference of spleen structure among C57BL/6NKorl, C57BL/6NA, and C57BL/6NB mice. Spleen tissues were stained with H&E. Upper line displayed at 100× magnification. Bottom line was viewed at 40× magnification. Arrow, Red pulp; arrow head, white pulp and lymphoid follicle.

• 

  Figure 3 The relative level of lymphocyte population in C57BL/6NKorl, C57BL/6NA, and C57BL/6NB mice. Histogram plots from flow cytometry represent the populations of T cells (left panel) and B cells (right panel) from spleen. Results are representative of 3 independent experiments.

• 

  Figure 4 Determination of cell-mediated immunotoxicity. After treatment with different Con A doses, the growth of cells was measured by using WST-1 assays. The data are presented as means±SD of three experiments. (A) and (B) panels was display responses of splenic T cells from C57BL/6NA and C57BL/6NB substrain, respectively. (C) The data presented the response of splenic T cells from C57BL/6NKorl substrain. *P<0.05 vs. med group. Med, media-treated group; Con A, concanavalin A.

• 

  Figure 5 Determination of humoral immunotoxicity. After treatment with different LPS doses, the growth of cells was measured by using WST-1 assays. The data are presented as the means±SD of the experiments. (A) and (B) panels display response of splenic B cell from two different source C57BL/6N substrain. (C) The data presented response of splenic B cells from C57BL/6NKorl substrain. *P<0.05 vs. med group. Med, media-treated group; LPS, lipopolysaccharide.

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