Lab Anim Res.  2017 Jun;33(2):132-139. 10.5625/lar.2017.33.2.132.

Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains

  • 1Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea.
  • 2College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
  • 3College of Pharmacy, Pusan National University, Busan 46241, Korea.
  • 4Exercise Biochemistry Laboratory, Korea National Sport University, Seoul 05541, Korea.
  • 5Department of Microbiology and Immunology, INJE University College of Medicine, Busan 47392, Korea.


Adaptive immunity is a type of immune response mediated by T and B cells, and is important response for immune response amplification and memory. In this study, the adaptive immunologic properties of C57BL/6NKorl substrain were compared with those of two other C57BL/6N substrains. There were no significant differences between the C57BL/6NKorl and the two other C57BL/6N substrains in the histological structures of the thymus and spleen, which are immunologic organs containing T cell and B cells. In addition, flow cytometric analysis did not reveal any significant differences in the distribution of T and B cell populations of the three substrains. To evaluate cell-mediated immunity of T cells in the three different substrains, we treated isolated T cells from spleen with Con A. The T cells of C57BL/6NKorl showed Con A-dependent proliferation of T cells at lower cell number than those in T cells from the other two C57BL/6N substrains. B cell-mediated humoral immune responses were not significant different among the three substrains. Thus, the results of this study provide evidence that C57BL/6NKorl mice are similar to those two other C57BL/6N substrains in humoral immunity, but C57BL/6NKorl has stronger response in cell mediated immunity.


C57BL/6N; C57BL/6NKorl; T cell; B cell; cell-mediated immunity; humoral immunity

MeSH Terms

Adaptive Immunity
Cell Count
Immunity, Cellular*
Immunity, Humoral
Thymus Gland
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