Korean J Parasitol.
2017 Dec;55(6):643-652. 10.3347/kjp.2017.55.6.643.
Molecular and Biochemical Characterization of Opisthorchis viverrini Calreticulin
- Affiliations
-
- 1Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani 12121, Thailand. rgrams@tu.ac.th
- 2Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
- 3Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
- KMID: 2403715
- DOI: http://doi.org/10.3347/kjp.2017.55.6.643
Abstract
- Calreticulin (CALR), a multifunctional protein thoroughly researched in mammals, comprises N-, P-, and C-domain and has roles in calcium homeostasis, chaperoning, clearance of apoptotic cells, cell adhesion, and also angiogenesis. In this study, the spatial and temporal expression patterns of the Opisthorchis viverrini CALR gene were analyzed, and calcium-binding and chaperoning properties of recombinant O. viverrini CALR (OvCALR) investigated. OvCALR mRNA was detected from the newly excysted juvenile to the mature parasite by RT-PCR while specific antibodies showed a wide distribution of the protein. OvCALR was localized in tegumental cell bodies, testes, ovary, eggs, Mehlis' gland, prostate gland, and vitelline cells of the mature parasite. Recombinant OvCALR showed an in vitro suppressive effect on the thermal aggregation of citrate synthase. The recombinant OvCALR C-domain showed a mobility shift in native gel electrophoresis in the presence of calcium. The results imply that OvCALR has comparable function to the mammalian homolog as a calcium-binding molecular chaperone. Inferred from the observed strong immunostaining of the reproductive tissues, OvCALR should be important for reproduction and might be an interesting target to disrupt parasite fecundity. Transacetylase activity of OvCALR as reported for calreticulin of Haemonchus contortus could not be observed.
Keyword
MeSH Terms
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Antibodies
Calcium
Calreticulin*
Cell Adhesion
Cell Body
Citrate (si)-Synthase
Eggs
Electrophoresis
Female
Fertility
Haemonchus
Homeostasis
In Vitro Techniques
Mammals
Molecular Chaperones
Opisthorchis*
Ovary
Ovum
Parasites
Prostate
Reproduction
RNA, Messenger
Testis
Vitellins
Antibodies
Calcium
Calreticulin
Citrate (si)-Synthase
Molecular Chaperones
RNA, Messenger
Vitellins
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