Clin Exp Vaccine Res.  2018 Jan;7(1):82-86. 10.7774/cevr.2018.7.1.82.

Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis

• Yu MA
• Jeong HW
• Park SJ
• Kim YI
• Kwon HI
• Kim EH
• Si YJ
• Yu KM
• Robles NJ
• Han HJ
• Choi YK

• KMID: 2402542
• DOI: http://doi.org/10.7774/cevr.2018.7.1.82

Abstract

To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.

Keyword

Severe fever with thrombocytopenia syndrome virus; Enzyme-linked immunosorbent assay; NP protein; Gn protein

MeSH Terms

Diagnosis*
Enzyme-Linked Immunosorbent Assay*
Fever*
Genotype
Nucleocapsid Proteins
Sensitivity and Specificity
Thrombocytopenia*
Nucleocapsid Proteins

Figure

• 

  Fig. 1 Detection of severe fever with thrombocytopenia syndrome virus (SFTSV) protein by ferret serum. (A) An r-NP and r-Gn based enzyme-linked immunosorbent assay was performed using the positive ferret serum CB1 and CB2. Positive samples demonstrated an optical density (OD) greater than 0.7. (B) After the infection of SFTSV, IFA was performed with CB1 and CB2 positive ferret serum. Two positive serums were detected over the 1:800. (C) Western blotting were performed with CB1 and CB2 proteins. First antibodies were used with CB1 positive serum and CB2 positive serum. M; protein marker; NP, nucleocapsid protein.

• 

  Fig. 2 Detection of severe fever with thrombocytopenia syndrome virus protein by ferret serum. (A) An r-NP and r-Gn based enzyme-linked immunosorbent assay (ELISA) was performed using the positive human serum. (B) An r-NP and r-Gn based ELISA was performed using the negative human serum. OD, optical density; NP, nucleocapsid protein.

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