Clin Exp Reprod Med.
2017 Dec;44(4):201-206. 10.5653/cerm.2017.44.4.201.
Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation
- Affiliations
-
- 1Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.
- 2Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. blasto@snubh.org
- 3Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.
- KMID: 2401622
- DOI: http://doi.org/10.5653/cerm.2017.44.4.201
Abstract
OBJECTIVE
The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure.
METHODS
Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and DGC. The percentages of sperm cells with fragmented DNA measured by the sperm chromatin dispersion test, normal sex chromosomes assessed by fluorescence in situ hybridization, and abnormal chromatin structure identified by toluidine blue staining were examined.
RESULTS
The percentage of sperm cells with fragmented DNA was significantly lower in the swim-up fraction (9.7%, p=0.001) than in the unprocessed fraction (27.0%), but not in the DGC fraction (27.8%, p=0.098). The percentage of sperm cells with normal X or Y chromosomes was comparable in the three fractions. The percentage of sperm cells with abnormal chromatin structure significantly decreased after DGC (from 15.7% to 10.3%, p=0.002). The swim-up method also tended to reduce the percentage of sperm cells with abnormal chromatin structure, but the difference was not significant (from 15.7% to 11.6%, p=0.316).
CONCLUSION
The swim-up method is superior for enriching genetically competent sperm.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Comparison between density gradient centrifugation method, an extended version of the horizontal swim up method and the combination of both for sperm selection
- Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation
- Specific tail swelling pattern in hypo-osmotic solution as a predictor of DNA fragmentation status in human spermatozoa
- Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting
- Effects of Semen Processing on Sperm Function: Differences between Swim-Up and Density Gradient Centrifugation
