Clin Exp Reprod Med.  2017 Dec;44(4):201-206. 10.5653/cerm.2017.44.4.201.

Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation

Affiliations
  • 1Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.
  • 2Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. blasto@snubh.org
  • 3Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.

Abstract


OBJECTIVE
The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure.
METHODS
Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and DGC. The percentages of sperm cells with fragmented DNA measured by the sperm chromatin dispersion test, normal sex chromosomes assessed by fluorescence in situ hybridization, and abnormal chromatin structure identified by toluidine blue staining were examined.
RESULTS
The percentage of sperm cells with fragmented DNA was significantly lower in the swim-up fraction (9.7%, p=0.001) than in the unprocessed fraction (27.0%), but not in the DGC fraction (27.8%, p=0.098). The percentage of sperm cells with normal X or Y chromosomes was comparable in the three fractions. The percentage of sperm cells with abnormal chromatin structure significantly decreased after DGC (from 15.7% to 10.3%, p=0.002). The swim-up method also tended to reduce the percentage of sperm cells with abnormal chromatin structure, but the difference was not significant (from 15.7% to 11.6%, p=0.316).
CONCLUSION
The swim-up method is superior for enriching genetically competent sperm.

Keyword

Aneuploidy; Density gradient centrifugation; DNA fragmentation; Sperm; Swim-up

MeSH Terms

Aneuploidy*
Centrifugation, Density Gradient*
Chromatin
DNA Fragmentation*
DNA*
Fluorescence
Humans
In Situ Hybridization
Infertility
Male
Methods
Semen
Sex Chromosomes*
Spermatozoa*
Tolonium Chloride
Y Chromosome
Chromatin
DNA
Tolonium Chloride
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