- Application of percoll density gradient centrifugation in seperating human X-and Y-bearing spermatozoa
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Kim JM, Lee SK, Suh BH, Lee JH, Chung KS
- KMID: 2259475
- Korean J Obstet Gynecol.
- 1993 Jul;36(7):1051-1057.
No abstract available. -
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- Purification of treponema pallidum from rabbit testicular tissue
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Lee MG, Cho SW, Lee KH, Lee JB
- KMID: 2231234
- Korean J Dermatol.
- 1991 Feb;29(1):56-64.
There are several different methods of purifying Treponema pallidum(TP) from rabbit testicular tissue. Among them, we compared the use of differential centrifugation, which has been most widely used, to Percoll... -
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- Separation of Human Epidermal Langerhans Cells by Density Gradient Centrifugation on a Colloidal Silica ( Percoll ) Gradient Method and Autologous , Allogeneic Mixed Skin Cell Leukocyte Culture Reactions
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Bae YJ, Haw CR
- KMID: 2146571
- Korean J Dermatol.
- 1987 Jun;25(3):299-308.
Separation of Langerhans cells in epidermis of 16 healthy Korean individuals were performcd. Separation of Langerhans cells by density gradient centrifugation on a colloidal sillica(percoll) polyvinilpyrrolidone gradient. And autologous, allogeneic... -
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- Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation
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Lee D, Jee BC
- KMID: 2469601
- Clin Exp Reprod Med.
- 2019 Mar;46(1):8-13.
- doi: 10.5653/cerm.2019.46.1.8
OBJECTIVE: Density gradient centrifugation (DGC) is frequently used to isolate high-motility fractions of spermatozoa. We compared the efficacy of four DGC media in terms of the percentage of morphologically normal... -
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- Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting
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Chi HJ, Kwak SJ, Kim SG, Kim YY, Park JY, Yoo CS, Park IH, Sun HG, Kim JW, Lee KH
- KMID: 2364656
- Clin Exp Reprod Med.
- 2016 Dec;43(4):199-206.
- doi: 10.5653/cerm.2016.43.4.199
OBJECTIVE: This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation... -
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- Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation
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Kim SW, Jee BC, Kim SK, Kim SH
- KMID: 2401622
- Clin Exp Reprod Med.
- 2017 Dec;44(4):201-206.
- doi: 10.5653/cerm.2017.44.4.201
OBJECTIVE: The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy,... -
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- Comparison of the effect of different media on the clinical outcomes of the density-gradient centrifugation/swim-up and swim-up methods
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Kim EK, Kim EH, Kim EA, Lee KA, Shin JE, Kwon H
- KMID: 1800422
- Clin Exp Reprod Med.
- 2015 Mar;42(1):22-29.
- doi: 10.5653/cerm.2015.42.1.22
OBJECTIVE: Sperm must be properly prepared in in vitro fertilization (IVF)-embryo transfer (ET) programs in order to control the fertilization rate and ensure that embryos are of high quality and... -
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- Isolation of Density Enrichment Fraction of Adipose-Derived Stem Cells from Stromal Vascular Fraction by Gradient Centrifugation Method
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Kim MK, Park YS, Park HS, Choi JM, Kim WJ, Park SE, Rhee EJ, Park CY, Lee WY, Oh KW, Park SW, Kim SW, Suh KS, Woo JT
- KMID: 2169015
- Endocrinol Metab.
- 2010 Jun;25(2):103-109.
- doi: 10.3803/EnM.2010.25.2.103
BACKGROUND: Adipose tissues include multipotent cells, the same as bone marrow-derived mesenchymal stem cells. The stromal vascular fractions (SVFs) from adipose tissues represent a heterogeneous cell population. The purpose of... -
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- Transmission electron microscopy for characterization of acrosomal damage after Percoll gradient centrifugation of cryopreserved bovine spermatozoa
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Oliveira L, Hossepian de Lima VFM, Levenhagen MA, dos Santos RM, Assumpcao , Jacomini J, de Andrade AFC, de Arruda , Beletti ME
- KMID: 1067399
- J Vet Sci.
- 2011 Sep;12(3):267-272.
- doi: 10.4142/jvs.2011.12.3.267
The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three... -
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- Comparision in the yield of fetal nucleated red blood cell between the first-and second-trimester using double density gradient centrifugation
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Jeon YJ, Kwon KH, Kim JW, Pang MG, Jung SC, Kim YJ
- KMID: 2273833
- Korean J Obstet Gynecol.
- 2010 Feb;53(2):127-136.
- doi: 10.5468/kjog.2010.53.2.127
OBJECTIVE: The aim of our study was to make a practical comparative evaluation of the first and second trimesters in order to determine the period during which a higher yield... -
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- Comparative Genetic Characterization of Plasmids of Agrobacterium Species Isolated in Korea
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Kim JH, Koo YB, Lee KY, Chung JK
- KMID: 2372976
- Yeungnam Univ J Med.
- 1984 Dec;1(1):41-48.
- doi: 10.12701/yujm.1984.1.1.41
The soil bacterium Agrobacterium tumefaciens is a plant pathogen that causes crown gall tumors by infecting the wounded dicotyledonous plants and subsequent integration of bacterial DNA into plant nuclear DNA.... -
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- Comparison of Intra-gall Bladder and Intra-common Bile Duct Injection of Collagenase Solution for Mouse Pancreatic Islet Isolation
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Park JK
- KMID: 2325112
- J Korean Surg Soc.
- 2007 Jul;73(1):8-12.
PURPOSE: A high yield of viable pancreatic islets is an essential prerequisite for the study of pancreatic islet transplantation. The purpose of this study is to compare the yield between... -
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- Specific tail swelling pattern in hypo-osmotic solution as a predictor of DNA fragmentation status in human spermatozoa
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Kim SW, Nho EJ, Lee JY, Jee BC
- KMID: 2469590
- Clin Exp Reprod Med.
- 2019 Dec;46(4):147-151.
- doi: 10.5653/cerm.2019.00458
OBJECTIVE: The aim of this study was to investigate DNA fragmentation status in human spermatozoa according to specific tail swelling patterns determined via hypo-osmotic swelling test (HOST). METHODS: Frozen semen samples... -
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- Chemosensitivity Test of Childhood Leukemic Cells
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Han TW, Jung SK, Kim HS, Kang CM
- KMID: 2335422
- J Korean Pediatr Soc.
- 1999 Aug;42(8):1111-1121.
PURPOSE: The purpose of the present study is to determine the relation between in vitro resistance to 9 drugs, measured with colorimetric methylthiazol tetrazolium(MTT) assay and prognostic factors. METHODS: Thirty... -
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- A New Prenatal Diagnosis of Fetal Cells Isolation from Maternal Peripheral Blood -Using Comparative Genomic Hybridization by Microdissection
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Yang YH, Kim SH, Kim SK, Park YW, Cho JS, Kim IK, Choi JR, Kim MS
- KMID: 2262620
- Korean J Obstet Gynecol.
- 2002 Feb;45(2):213-219.
OBJECTIVE: The objective of this study was to determine the clinical use of CGH (comparative genomic hybridization) for detection of fetal aneuploidy from fetal cells (nucleated red blood cells, nRBCs)... -
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- Osteogenic Differentiation of Circulating Peripheral Blood Derived Mesenchymal Progenitor Cells
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Eun SC, Kim JH, Heo CY, Baek RM, Chang H, Minn KW
- KMID: 2144712
- J Korean Soc Plast Reconstr Surg.
- 2008 May;35(3):229-234.
PURPOSE: There are some reports presenting that peripheral blood contain circulating hematopoietic cells as well as, in significantly smaller quantities, mesenchymal stem cells. The purposes of this study is to... -
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- Effect of Uric Acid (UA) on C-reactive Protein (CRP) Expression in Peripheral Blood Mononuclear Cells (PBMC)
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Kang DH, Yu M, Ryu JH
- KMID: 2253704
- Korean J Nephrol.
- 2007 Nov;26(6):669-676.
PURPOSE: Systemic inflammatory reaction (SIR) is an important determinant of cardiovascular (CV) mortality in CRF patients. UA is an end-product of purine metabolism, and recent studies have demonstrated that an... -
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- Isolation, Characterization and Tri-lineage Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord Blood
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Yang SE, Yang YS, Ha CW
- KMID: 2399120
- J Korean Orthop Assoc.
- 2004 Sep;39(5):537-545.
PURPOSE: Human umbilical cord blood (hUCB) is well known for a good source of hematopoietic stem cells (HSCs). However, the presence of mesenchymal stem cells (MSCs) in hUCB is still... -
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- In vitro Immunization of Human Tonsilar Lymphocytes Using Slice Culture Sytem
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Park SS, Lee HL, Seoh JY, Hong SK, Han JJ, Won T, Bae JM, Shin MH, Lee HC
- KMID: 2283073
- Korean J Phys Anthropol.
- 1998 Jun;11(1):131-138.
It is difficult to immunize human lymphocytes in vitro by conventional cell culture methods. Activation of lymphocytes requires not only specific antigen stimulation but also delicate cell to cell interaction.... -
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- Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-kappaB in Acute Transection Spinal Cord Injury in Rats
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Shrestha R, Qiao JM, Shen FG, Bista KB, Zhao ZN, Yang J
- KMID: 2018094
- J Korean Neurosurg Soc.
- 2014 Nov;56(5):375-382.
- doi: 10.3340/jkns.2014.56.5.375
OBJECTIVE: To assess the effect of bone marrow mononuclear cells (BMMNCs) transplantation in the expression of nuclear factor-kappaB (NF-kappaB) in spinal cord injury (SCI) in rats. METHODS: BMMNCs were isolated from... -
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