Korean J Obstet Gynecol.
2010 Feb;53(2):127-136. 10.5468/kjog.2010.53.2.127.
Comparision in the yield of fetal nucleated red blood cell between the first-and second-trimester using double density gradient centrifugation
- Affiliations
-
- 1Department of Biochemistry, Ewha Womans University, School of Medicine, Seoul, Korea.
- 2GenDix Inc., Seoul, Korea.
- 3Biomedlab Co., Seoul, Korea.
- 4Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Gyeonggi-Do, Korea.
- 5Department of Obstetrics and Gynecology, Ewha Womans University, School of Medicine, Seoul, Korea.
- 6Medical Research Institute, Ewha Womans University, Seoul, Korea. kkyj@ewha.ac.kr
- KMID: 2273833
- DOI: http://doi.org/10.5468/kjog.2010.53.2.127
Abstract
OBJECTIVE
The aim of our study was to make a practical comparative evaluation of the first and second trimesters in order to determine the period during which a higher yield of fetal nucleated red blood cells (FNRBCs) can be obtained.
METHODS
NRBCs were isolated from maternal blood during the first and second trimesters of pregnancy using double Percoll gradients with different osmolarities. Magnetic activated cell sorting was performed with Kleihauer-Betke stain. We isolated fetal NRBCs from 10 mL of samples of maternal blood and determined fetal sex and fetal aneuploidy by fluorescence in situ hybridization (FISH).
RESULTS
The average number of NRBCs was 9.85 in samples obtained during the first trimester and 14.88 in samples obtained during the second trimester (P=0.07). The average number of NRBCs with Y chromosome signals was 5.73 in the first trimester and 8.22 in second trimester (P=0.56). However, the percentage of NRBCs with Y chromosome signals in the first trimester (70.6%) was significantly higher than in the second trimester (59.8%) (P=0.049). We diagnosed the blood samples from 7 pregnant women having fetal aneuploidy using this method and the number of NRBCs was 18.4.
CONCLUSION
The method using Percoll osmolarity and a double density gradient system may be a very useful method for separation of NRBCs in the first trimester of pregnancy and also in the second trimester.
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