Korean J Vet Res.
2017 Dec;57(4):215-222. 10.14405/kjvr.2017.57.4.215.
Temporal and subcellular distributions of Cy5.5-labeled hyaluronic acid nanoparticles in mouse organs during 28 days as a drug carrier
- Affiliations
-
- 1College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130-118, China.
- 2College of Veterinary Medicine and Veterinary Medical Center, Chungbuk National University, Cheongju 28644, Korea. ywyun@cbu.ac.kr, synam@cbu.ac.kr
- 3Asan Institute for Life Sciences, Asan Medical Center and University of Ulsan, Seoul 05505, Korea.
- KMID: 2401290
- DOI: http://doi.org/10.14405/kjvr.2017.57.4.215
Abstract
- Temporal and subcellular distributions of hyaluronic acid (HA) as a degradable nanoparticle (NP) in animals were investigated to determine if HA-NP could be utilized as an appropriate drug delivery system. After mice were intravenously injected with 5 mg/kg of Cy5.5-labeled HA-NP sized 350-400 nm or larger HA-polymers, the fluorescence intensity was measured in all homogenized organs from 0.5 h to 28 days. HA-NP was greatly detected in spleen, liver and kidney until day 28, while it was maintained at low levels in other organs. HA-polymer was observed at low levels in all organs. HA-NP quantities in spleen and liver were reduced until day 3, but increased sharply between days 3 and 7, then decreased again, while their HA-polymers were maintained at low levels until day 28. In kidneys, both HA-NP and HA-polymer showed high levels after 0.5 h of administration, but steadily decreased until day 28. According to ultrastructural analyses, HA-NP was engulfed in Kupffer cells of liver and macrophages of spleen and kidney at day 1 and was accumulated in the cytoplasm of kidney tubular cells at day 7. Overall, these findings suggest that HA-NP could be considered a desirable drug carrier in the liver, kidney, or spleen.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Distribution and accumulation of Cy5.5-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles in the tissues of ICR mice
- Long-circulating and target-specific distributions of cyanine 5.5-labeled hyaluronic acid nanoparticles in mouse organs during 28 days after a single administration
- Comparative in vivo biodistributions of nanoparticles and polymers of ¹â·â·lutetium-labeled hyaluronic acids in mice during 28 days
- Distribution and accumulation of 177Lu-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles in the tissues of ICR mice
- Hyaluronidase: An overview of its properties, applications, and side effects
