J Vet Sci.  2013 Dec;14(4):473-479. 10.4142/jvs.2013.14.4.473.

Distribution and accumulation of Cy5.5-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles in the tissues of ICR mice

• Hue JJ
• Lee HJ
• Jon S
• Nam SY
• Yun YW
• Kim JS
• Lee BJ

• KMID: 1712315
• DOI: http://doi.org/10.4142/jvs.2013.14.4.473

Abstract

Free Cy5.5 dye and Cy5.5-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION) have been routinely used for in vivo optical imaging. However, there is little information about the distribution and accumulation of free Cy5.5 dye and Cy5.5-labeled TCL-SPION in the tissues of mice. Free Cy5.5 dye (0.1 mg/kg body weight) and Cy5.5-labeled TCL-SPION (15 mg/kg body weight) were intravenously injected into the tail vein of ICR mice. The biodistribution and accumulation of the TCL-SPION and Cy5.5 were observed by ex vivo optical imaging and fluorescence signal generation at various time points over 28 days. Cy5.5 dye fluorescence in various organs was rapidly eliminated from 0.5 to 24 h post-injection. Fluorescence intensity of Cy5.5 dye in the liver, lung, kidney, and stomach was fairly strong at the early time points within 1 day post-injection. Cy5.5-labeled TCL-SPION had the highest fluorescence density in the lung at 0.5 h post-injection and decreased rapidly over time. Fluorescence density in liver and spleen was maintained over 28 days. These results suggest that TCL-SPION can be useful as a carrier of therapeutic reagents to treat diseases by persisting for long periods of time in the body.

Keyword

accumulation; biodistribution; Cy5.5 dye; thermally cross-linked superparamagnetic iron oxide nanoparticles; toxicity

MeSH Terms

Animals
Carbocyanines/*pharmacology
Ferric Compounds/*pharmacology
Fluorescent Dyes/*pharmacology
Kinetics
Male
Mice
Mice, Inbred ICR
Nanoparticles/*metabolism
Time Factors
Tissue Distribution
Carbocyanines
Ferric Compounds
Fluorescent Dyes

Figure

• 

  Fig. 1 The location of various organs used for kinetic studies of free Cy5.5 and Cy5.5-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION). B: brain, Ly: lymph node, Th: thymus, H: heart, Lu: lung, LI: large intestine, Li: liver, SI: small intestine, Sp: spleen, St: stomach, K: kidney, AG: adrenal gland, Te: testis, Ep: epididymis.

• 

  Fig. 2 Florescence images showing the biodistribution and accumulation of fluorescence in various organs for 28 days after the injection of 0.1 mg/kg Cy5.5. Yellow indicates a higher intensity at 615~707 nm. Images were acquired with an exposure time of 1 sec using the Cy5.5 filter channel. Left: organs of a normal mouse; Middle and right: organs of mice injected with Cy5.5.

• 

  Fig. 3 Florescence images showing the biodistribution and accumulation of fluorescence in various organs over a 28-day period after the injection of 15 mg/kg Cy5.5-labeled TCL-SPION. Yellow indicates higher intensity at 615~707 nm. Images were acquired with an exposure time of 1 sec using the Cy5.5 filter channel. Left: organs of a normal mouse; Middle and right: organs of mice injected with Cy5.5-labeled TCL-SPION.

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