J Korean Assoc Oral Maxillofac Surg.
2009 Jun;35(3):137-145.
Bone Regeneration with MMP sensitive hyaluronic acid-based hydrogel, rhBMP-2 and nanoparticles in rat calvarial critical size defect(CSD) model
- Affiliations
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- 1Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Korea. leejongh@snu.ac.kr
- 2Dental Research Institute, School of Dentistry, Seoul National University, Korea.
- 3Department of Materials Science and Engineering, Gwangju Institute of Science and Technology, Korea.
- 4Department of Biomedical Engineering, Biomedical Engineering, Brain Korea 21 2nd Project for Biomedical Science, Korea Artificial Organ Center, College of Medicine, Korea University, Korea.
- 5Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Korea.
Abstract
- As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase( MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model.Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel- Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity. These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.
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