J Korean Acad Periodontol.  2009 Mar;39(1):77-86. 10.5051/jkape.2009.39.1.77.

Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects

• Kwon SH
• Lim HC
• Choi KH
• Kim MS
• Lee JH
• Jung UW
• Yun JH
• Kim CS
• Choi SH
• Cho KS

• KMID: 1783519
• DOI: http://doi.org/10.5051/jkape.2009.39.1.77

Abstract

PURPOSE: Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system.
MATERIALS AND METHODS
Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals.
RESULTS
The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks.
CONCLUSION
rhBMP-2 produced by E.coli expression system may be effective for bone regeneration.

Keyword

bone morphogenetic protein-2; E.coli expression system; Absorbable collagen sponge; rat calvarial defect

MeSH Terms

Animals
Bone Regeneration
Collagen
Durapatite
Escherichia
Escherichia coli
Humans
Male
Osteogenesis
Rats
Rats, Sprague-Dawley
Salicylamides
Collagen
Durapatite
Salicylamides

Figure

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  Figure 1 Schematic diagram of the calvarial osteotomy defect showing the histometric analysis

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  Figure 2 Representative photomicrographs of defect site receiving the sham surgery at 2weeks(arrow heads : defect margin ; HE stain, magnification × 10).

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  Figure 3 Representative photomicrographs of defect site receiving the sham surgery at 8weeks(arrow heads : defect margin ; HE stain, magnification × 10).

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  Figure 4 Representative photomicrographs of defect site receiving the positive control at 2weeks(arrow heads : defect margin ; HE stain, magnification × 10).

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  Figure 5 Representative photomicrographs of defect site receiving the positive control at 8weeks(arrow heads : defect margin ; HE stain, magnification × 10).

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  Figure 6 Representative photomicrographs of defect site receiving the experimental at 2weeks(arrow heads:defect margin ; HE stain, magnification × 10).

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  Figure 7 Representative photomicrographs of defect site receiving the experimental at 2weeks(arrow heads:defect margin, PB:pre-existing bone, NB:new bone ; HE stain, magnification × 100).

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  Figure 8 Representative photomicrographs of defect site receiving the experimental at 2weeks(central portion ; HE stain, magnification × 100)

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  Figure 9 Representative photomicrographs of defect site receiving the experimental at 8weeks(arrow heads: defect margin ; HE stain, magnification × 10).

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  Figure 10 Representative photomicrographs of defect site receiving the experimental at 8weeks(arrow heads:defect margin, PB:pre-existing bone, NB:new bone ; HE stain, magnification × 100).

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  Figure 11 Representative photomicrographs of defect site receiving the experimental at 8weeks(central portin; HE stain, magnification × 100).

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