J Korean Acad Periodontol.  2008 Aug;38(Suppl):325-334. 10.5051/jkape.2008.38.Suppl.325.

Effect of MBCP block as carrier of rhBMP-2 in combination with ePTFE membrane on bone formation in rat calvarial defects

• Shin CW
• Cho KS
• Jung SW
• Kim CS
• Choi SH
• Yun JH

• KMID: 1486867
• DOI: http://doi.org/10.5051/jkape.2008.38.Suppl.325

Abstract

PURPOSE
The carrier used as delivery agent for bone morphogenetic proteins(BMPs) should also act as a scaffold for new bone formation. Moreover, bone formation should be predictable in terms of the volume and shape. This study examined the osteogenic effect of macroporous biphasic calcium phosphate (MBCP) block combined with ePTFE membrane as a carrier for recombinant human bone morphogenetic proteins (rhBMP-2). In addition, the additive effect of ePTFE membrane on bone formation was evaluated.
MATERIALS AND METHODS
Eight-millimeter critical sized calvarial defects were created surgically in 28 male Sprague-Dawley rats. The animals were divided into 2 groups containing 14 animals each. The defects were treated with either rhBMP-2/MBCP block (rhBMP-2/MBCP group) or rhBMP-2/MBCP block/ePTFE membrane (rhBMP-2/MBCP/ePTFE group). A disc-shaped MBCP block (3 mm height and 8 mm diameter) was used as the carrier for the rhBMP-2 and ePTFE membrane was used to cover the rhBMP-2/MBCP block. The histologic and histometric parameters were used to evaluate the defects after 2- or 8-week healing period (7 animals/group/healing interval).
RESULTS
The level of bone formation in the defects of both groups was significantly higher at 8 weeks than that at 2 weeks (P < 0.05). The ePTFE membrane has no additional effect compared with the rhBMP-2/MBCP block only. However, at 8 weeks, rhBMP-2/MBCP/ePTFE group showed more even bone formation on the top of the MBCP block than the rhBMP-2/MBCP group.
CONCLUSION
These results suggest that the ePTFE membrane has no additive effect on bone formation when a MBCP block is used as a carrier for rhBMP-2.

Keyword

rhBMP-2; MBCP block; ePTFE membrane; carrier

MeSH Terms

Animals
Bone Morphogenetic Proteins
Calcium
Durapatite
Humans
Hydroxyapatites
Male
Membranes
Osteogenesis
Rats
Rats, Sprague-Dawley
Bone Morphogenetic Proteins
Calcium
Durapatite
Hydroxyapatites