J Korean Acad Periodontol.  2008 Aug;38(Suppl):355-362. 10.5051/jkape.2008.38.Suppl.355.

rhBMP-2 using biphasic calcium phosphate block as a carrier induces new bone formation in a rat subcutaneous tissue

• Kim JI
• Yun JH
• Chae GJ
• Jung SW
• Kim CS
• Cho KS

• KMID: 1486870
• DOI: http://doi.org/10.5051/jkape.2008.38.Suppl.355

Abstract

PURPOSE
The carrier for the delivery of bone morphogenetic proteins(BMPs) should also serve as a scaffold for new bone growth. In addition, predictable bone formation in terms of the volume and shape should be guaranteed. This study evaluated the ectopic bone formation of recombinant human BMP-2(rhBMP-2) using a micro macroporous biphasic calcium phosphate (MBCP: mixture of betaTCP and HA) block as a carrier in a rat subcutaneous assay model.
MATERIALS AND METHODS
Subcutaneous pockets were created on the back of 40 male Sprague-Dawley rats. In the pockets, rhBMP-2/MBCP and MBCP alone were implanted. The blocks were evaluated by histological and histometric parameters after a healing interval of 2 weeks (each 10 rats; MBCP and rhBMP-2/MBCP) or 8 weeks (each 10 rats; MBCP and rhBMP-2/MBCP).
RESULTS
The shape and volume of the block was maintained stable over the healing period. No histological bone forming activity was observed in the MBCP alone sites after 2 weeks and there was minimal new bone formation at 8 weeks. In the rhBMP-2/MBCP sites, new bone formation was evident in the macropores of the block. The new bone area at 8 weeks was greater than at 2 weeks. There was a further increase in the quantity of new bone with the more advanced stage of remodeling.
CONCLUSIONS
A MBCP block could serve as a carrier system for predictable bone tissue engineering using rhBMPs.

Keyword

rhBMP-2; biphasic calcium phosphate block; carrier; ectopic bone formation

MeSH Terms

Animals
Bone and Bones
Bone Development
Calcium
Humans
Hydroxyapatites
Male
Osteogenesis
Rats
Rats, Sprague-Dawley
Subcutaneous Tissue
Calcium
Hydroxyapatites