Tissue Eng Regen Med.
2017 Jun;14(3):297-306. 10.1007/s13770-017-0038-1.
Oligomeric Procyanidins (OPCs) Inhibit Procollagen Type I Secretion of Fibroblasts
- Affiliations
-
- 1Department of Plastic and Reconstructive Surgery, College of Medicine, Seoul National University, 103 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
- 2Faceplus Aesthetic Clinic, 856 Eonju-ro, Gangnam-gu, Seoul 06017, Korea.
- 3Hana Academy Seoul, 535 Yeonseo-ro, Eunpyeong-gu, Seoul 03305, Korea.
- 4Department of Internal Medicine, School of Medicine, Yeungnam University, 170 Hyeonchung-ro, Nam-gu, Daegu 42415, Korea.
- 5Department of Internal Medicine, Keimyung University Dongsan Medical Center, 56 Dalseong-ro, Daegu 41931, Korea.
- 6Department of Rehabilitation Medicine, Chungnam National University Hospital, 282 Munhwa-ro, Jung-gu, Daejeon 35015, Korea.
- 7Department of Biomedical Engineering, College of Medicine, Seoul National University, 103 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
- 8Department of Rehabilitation Medicine, Dankook University Hospital, 119 Dandae-ro, Cheonan 31116, Korea.
- 9Department of Rehabilitation Medicine, College of Medicine, Seoul National University, 103 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
- 10Department of Plastic and Reconstructive Surgery, Institute of Human Environment Interface Biology, College of Medicine, Seoul National University, 103 Daehak-ro, Jongno-gu, Seoul 03080, Korea. psthchoi@snu.ac.kr
- 11Department of Plastic and Reconstructive Surgery, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
- KMID: 2395086
- DOI: http://doi.org/10.1007/s13770-017-0038-1
Abstract
- Wound healing is composed of a complex process that requires harmonies of various cell populations where fibroblasts play the main role. Oligomeric procyanidins (OPC) are main components of grape (Vitis vinifera) seed extracts, and recent studies showed OPC's effects on inflammation, cell migration, and proliferation. We investigated the effect of OPC on fibroblasts to regulate wound healing process. Human dermal fibroblast known as Hs27 cells were treated with various concentrations of OPC (0, 2.5, 5, 10, and 20 µg/µl). Cell cytotoxicity was evaluated by the Cell Counting Kit assay, and the expression levels of secreted procollagen were analyzed. Procollagen levels in OPC treated cells exposed to transforming growth factor beta 1 (TGF-β1) or ascorbic acid were evaluated using Western blot and immunocytochemistry. Relative mRNA expressions of procollagen, molecular chaperone such as HSP47, P4H were determined by real-time PCR in OPC treated cells. OPC showed no cytotoxicity on Hs27 cells at every concentration but inhibited procollagen secretion in a dose-dependent manner. The inhibitory effect also appeared under TGF-β1 induced collagen overproduction. Immunocytochemistry showed that higher levels of intracytoplasmic procollagen were accumulated in TGF-β1 treatment group, whereas ascorbic acid induced a release of accumulated procollagen under OPC treatment. The mRNA expressions of procollagen, molecular chaperone were not affected by OPC, but procollagen level was increased when exposed to TGF-β1. OPC inhibits procollagen secretion from fibroblasts with no effects on cell proliferations even under the environment of TGF-b1-induced collagen overproduction. OPC could regulate the diseases and symptoms of abnormal overabundant collagen production.
MeSH Terms
-
Ascorbic Acid
Blotting, Western
Cell Count
Cell Movement
Collagen
Collagen Type I*
Fibroblasts*
Humans
Immunohistochemistry
Inflammation
Molecular Chaperones
Proanthocyanidins*
Procollagen*
Real-Time Polymerase Chain Reaction
RNA, Messenger
Transforming Growth Factor beta
Vitis
Wound Healing
Ascorbic Acid
Collagen
Collagen Type I
Molecular Chaperones
Proanthocyanidins
Procollagen
RNA, Messenger
Transforming Growth Factor beta
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