Tissue Eng Regen Med.  2017 Oct;14(5):587-593. 10.1007/s13770-017-0066-x.

Differentiation Potential of Breast Milk-Derived Mesenchymal Stem Cells into Hepatocyte-Like Cells

• Sani M
• Ebrahimi S
• Aleahmad F
• Salmannejad M
• Hosseini S
• Mazarei G
• Talaei-Khozani T

• KMID: 2394992
• DOI: http://doi.org/10.1007/s13770-017-0066-x

Abstract

Human breast milk stem cells (hBSCs) contain a population of cells with the ability to differentiate into various cell lineages for cell therapy applications. The current study examined the differentiation potential of hBSCs into hepatocytes- like cells. The cells were isolated from the breast milk and were treated with hepatogenic medium containing hepatocyte growth factor, insulin-like growth factor and dexamethasone for 7 days subsequently; Oncostatin M was added to the culture media. RT-PCR and immunocytochemistry were performed to detect the hepatogenic markers. The glycogen storage and the ability of the cells to absorb and release indocynanin green were also tested. The data showed that most of the differentiated cells formed cell aggregates after the 30th day, with more cells accumulated to form spheroids. RT-PCR revealed the expression of the hepatic nuclear factor, albumin, cytokeratin 18 and 19, cytochrome P2B6, glucose-6-phospahtase and claudin. The functional assays also showed glycogen storage and omission of indicynine green. Our study demonstrated hBSCs are novel population that can differentiate into hepatocyte-like cells.

Keyword

Cell culture; Differentiation; Hepatocyte; Human breast milk; Stem cell

MeSH Terms

Breast*
Cell Culture Techniques
Cell Lineage
Cell- and Tissue-Based Therapy
Culture Media
Cytochromes
Dexamethasone
Glycogen
Hepatocyte Growth Factor
Hepatocytes
Humans
Immunohistochemistry
Keratin-18
Mesenchymal Stromal Cells*
Milk, Human
Oncostatin M
Stem Cells
Culture Media
Cytochromes
Dexamethasone
Glycogen
Hepatocyte Growth Factor
Keratin-18
Oncostatin M