Korean J Vet Res.  2017 Jun;57(2):117-126. 10.14405/kjvr.2017.57.2.117.

Identification of microbiome with 16S rRNA gene pyrosequencing and antimicrobial effect of egg white in bovine mastitis

Affiliations
  • 1Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. kwonhj01@snu.ac.kr
  • 2Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
  • 3Farm Animal Clinical Training and Research Center, Institutes of GreenBio Science Technology, Pyeongchang 25354, Korea.
  • 4Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.

Abstract

Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram-positive pathogens (Enterococcus faecalis 37% and Staphylococcus aureus 36%) were higher than that of a Gram-negative pathogen (Escherichia coli 15%). Resistance frequencies to ampicillin and norfloxacin were lowest in Staphylococcus aureus (21%), Enterococcus faecalis (23%), and Escherichia coli (33%), and the antimicrobial activity of chicken egg white was higher than those of lysozyme and duck egg white. Pyrosequencing results revealed clear differences between the microbiomes of mastitic and normal raw milk samples and revealed a slightly similar, but clearly different, composition of pathogens compared to that from the culture method. Thus, pyrosequencing may be useful for elucidating changes in microbiomes during mastitis progression and treatment. A chicken egg white and antibiotic combination may help with mastitis treatment; however, further studies are needed.

Keyword

antibiotic resistance; bovine mastitis; microbial pathogens; microbiome; raw milk

MeSH Terms

Agriculture
Ampicillin
Animals
Cattle
Chickens
Drug Resistance, Microbial
Ducks
Egg White*
Enterococcus faecalis
Escherichia coli
Female
Genes, rRNA*
Mastitis
Mastitis, Bovine*
Methods
Microbiota*
Milk
Muramidase
Norfloxacin
Ovum*
Staphylococcus aureus
Ampicillin
Muramidase
Norfloxacin
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