J Vet Sci.  2011 Mar;12(1):95-97. 10.4142/jvs.2011.12.1.95.

Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates

• Oliveira M
• Bexiga R
• Nunes SF
• Vilela CL

• KMID: 1067343
• DOI: http://doi.org/10.4142/jvs.2011.12.1.95

Abstract

Staphylococcus (S.) aureus is a common infectious agent of bovine chronic mastitis, a disease that is difficult to eradicate. The abilities of Staphylococci to be internalized and form a biofilm can contribute to host immunological defence evasion that subsequently impairs antimicrobial therapy. The invasive capability of six S. aureus field isolates with different biofilm-forming profiles was compared in vitro using a bovine mammary epithelial cell line. This was further confirmed in primary cell cultures using fluorescent rRNA probes against S. aureus. The results suggest that S. aureus invasion levels are not related to biofilm formation.

Keyword

biofilm; bovine mammary epithelial cells; bovine subclinical mastitis; invasion; Staphylococci

MeSH Terms

Animals
*Biofilms
Cattle
Cell Line
Colony Count, Microbial/veterinary
Epithelial Cells/microbiology
Female
In Situ Hybridization, Fluorescence
Mastitis, Bovine/*microbiology
Portugal
Staphylococcal Infections/*veterinary
Staphylococcus aureus/classification/genetics/immunology/*physiology
Virulence Factors/i

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