J Vet Sci.  2011 Mar;12(1):95-97. 10.4142/jvs.2011.12.1.95.

Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates

Affiliations
  • 1CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, 1300-477 Lisbon, Portugal. moliveira@fmv.utl.pt

Abstract

Staphylococcus (S.) aureus is a common infectious agent of bovine chronic mastitis, a disease that is difficult to eradicate. The abilities of Staphylococci to be internalized and form a biofilm can contribute to host immunological defence evasion that subsequently impairs antimicrobial therapy. The invasive capability of six S. aureus field isolates with different biofilm-forming profiles was compared in vitro using a bovine mammary epithelial cell line. This was further confirmed in primary cell cultures using fluorescent rRNA probes against S. aureus. The results suggest that S. aureus invasion levels are not related to biofilm formation.

Keyword

biofilm; bovine mammary epithelial cells; bovine subclinical mastitis; invasion; Staphylococci

MeSH Terms

Animals
*Biofilms
Cattle
Cell Line
Colony Count, Microbial/veterinary
Epithelial Cells/microbiology
Female
In Situ Hybridization, Fluorescence
Mastitis, Bovine/*microbiology
Portugal
Staphylococcal Infections/*veterinary
Staphylococcus aureus/classification/genetics/immunology/*physiology
Virulence Factors/i

Reference

1. Almeida RA, Matthews KR, Cifrian E, Guidry AJ, Oliver SP. Staphylococcus aureus invasion of bovine mammary epithelial cells. J Dairy Sci. 1996. 79:1021–1026.
2. Almeida RA, Oliver SP. Interaction of coagulase-negative Staphylococcus species with bovine mammary epithelial cells. Microb Pathog. 2001. 31:205–212.
Article
3. Barkema HW, Schukken YH, Zadoks RN. Invited Review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. J Dairy Sci. 2006. 89:1877–1895.
Article
4. Berlutti F, Morea C, Battistoni A, Sarli S, Cipriani P, Superti F, Ammendolia MG, Valenti P. Iron availability influences aggregation, biofilm, adhesion and invasion of Pseudomonas aeruginosa and Burkholderia cenocepacia. Int J Immunopathol Pharmacol. 2005. 18:661–670.
Article
5. Bradley AJ, Leach KA, Breen JE, Green LE, Green MJ. Survey of the incidence and aetiology of mastitis on dairy farms in England and Wales. Vet Rec. 2007. 160:253–257.
Article
6. Dziewanowska K, Patti JM, Deobald CF, Bayles KW, Trumble WR, Bohach GA. Fibronectin binding protein and host cell tyrosine kinase are required for internalization of Staphylococcus aureus by epithelial cells. Infect Immun. 1999. 67:4673–4678.
Article
7. Ferens WA, Bohach GA. Persistence of Staphylococcus aureus on mucosal membranes: superantigens and internalization by host cells. J Lab Clin Med. 2000. 135:225–230.
Article
8. Fitzpatrick JL, Mayer SJ, Vilela C, Bland PW, Stokes CR. Cytokine-induced major histocompatibility complex class II antigens on cultured bovine mammary gland epithelial cells. J Dairy Sci. 1994. 77:2940–2948.
Article
9. Hensen SM, Pavičić MJAMP, Lohuis JACM, de Hoog JAM, Poutrel B. Location of Staphylococcus aureus within the experimentally infected bovine udder and the expression of capsular polysaccharide type 5 in situ. J Dairy Sci. 2000. 83:1966–1975.
Article
10. Hensen SM, Pavičić MJAMP, Lohuis JACM, Poutrel B. Use of bovine primary mammary epithelial cells for the comparison of adherence and invasion ability of Staphylococcus aureus strains. J Dairy Sci. 2000. 83:418–429.
Article
11. Latasa C, Roux A, Toledo-Arana A, Ghigo JM, Gamazo C, Penadés JR, Lasa I. BapA, a large secreted protein required for biofilm formation and host colonization of Salmonella enterica serovar Enteritidis. Mol Microbiol. 2005. 58:1322–1339.
Article
12. Oliveira M, Bexiga R, Nunes SF, Carneiro C, Cavaco LM, Bernardo F, Vilela CL. Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates. Vet Microbiol. 2006. 118:133–140.
Article
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