Korean J Vet Res.  2018 Mar;58(1):51-55. 10.14405/kjvr.2018.58.1.51.

Molecular identification of coagulase-negative staphylococci by rpoB sequence typing

• Seong WJ
• Kim D
• Kim EK
• Ko DS
• Ro Y
• Kim JH
• Kwon HJ

• KMID: 2437678
• DOI: http://doi.org/10.14405/kjvr.2018.58.1.51

Abstract

Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.

Keyword

16S rRNA gene sequencing; bovine mastitis; coagulase-negative staphylococci; rpoB sequence typing

MeSH Terms

Animals
Bacterial Infections
Cattle
Diagnosis
Epidemiology
Female
Genes, rRNA
Mastitis, Bovine
Methods