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Classification of Borrelia burgdorferi Sensu Lato Isolated in Korea by Restriction Fragment Length Polymorphism

Park KH, Hahn MJ, Lee SH, Kim JH

  • KMID: 2099280
  • J Korean Soc Microbiol.
  • 1998 Apr;33(2):119-128.
The organization of the ribosomal genes is unique in Borrelia burgdorferi in that the rrl (23S) and rrf (SS) genes are duplicated in tandem. Twelve Korean Borrelia isolates were classified...
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A Case of Bacteremia Due to Microbacterium oleivorans Identified by 16S rRNA Sequencing Analysis

Kim BH, Lee MK

Microbacterium oleivorans is a gram-positive, coryneform rod bacterium. The pathogenic potential of the Microbacterium species has recently been reported to be increasing. Microbacterium comprises approximately 50 species. The differences in...
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Detection of 23S rRNA Mutation Associated with Clarithromycin Resistance in Children with Helicobacter pylori Infection

Ko JS, Yang HR, Seo JK

  • KMID: 2275195
  • Korean J Pediatr Gastroenterol Nutr.
  • 2004 Sep;7(2):137-142.
PURPOSE: The resistance of H. pylori to clarithromycin is one of the major causes of eradication failure. In H. pylori, clarithromycin resistance is due to point mutation in 23S rRNA....
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Basic Concepts of Bacterial Taxonomy

Kim YS, Jang SJ

The three components of taxonomy are classification, nomenclature and identification. Traditionally, bacterial classification and identification were performed based on the morphology and the biochemical data of the bacteria. In newer...
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A Case of Klebsiella pneumoniae Unidentified by Conventional Biochemical Tests

Kim YC, Kim JS, Park JY, Kim HS, Song W, Kang SH, Cho HC, Lee KM

  • KMID: 1517780
  • J Lab Med Qual Assur.
  • 2004 Dec;26(2):239-242.
A sixty-seven-year-old man was admitted to a hospital with symptoms of high fever and chill. Bacterial isolates were obtained from sputum and blood. These isolates were identified as Klebsiella terrigena...
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Sequence Analysis of the Internal Transcribed Spacer of Ribosomal DNA in the Genus Rhizopus

Park YJ, Min BR

  • KMID: 2312459
  • Mycobiology.
  • 2005 Jun;33(2):109-112.
The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by...
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Detection of genetic mutations associated with macrolide resistance of Mycoplasma pneumoniae

Oh CE, Choi EH, Lee HJ

PURPOSE: The aim of this study was to identify mutations associated with macrolide resistance in Mycoplasma pneumoniae (MP) and to establish a cultural method to determine antimicrobial susceptibility. METHODS: Nasopharyngeal aspirates...
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Identification of Gemella species by 16S ribosomal RNA gene sequencing from two patients with infective endocarditis

Sohn KM, Ko KS, Kim J, Rhee JY, Oh WS, Peck KR, Song JH

  • KMID: 2306002
  • Korean J Med.
  • 2006 May;70(5):591-596.
Gemella species are opportunistic pathogens, which cause endocarditis, meningitis, musculoskeletal infections, or pulmonary infections. It is difficult to identify all strains of these species by a conventional culture system. Because...
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A novel PCR primers HPU185 and HPL826 based on 16S rRNA gene for detection of Helicobacter pylori

Kim JB, Kim GH, Kim H, Jin HS, Kim YS, Ha SH, Lee DK

  • KMID: 2146872
  • J Korean Soc Microbiol.
  • 2000 Aug;35(4):283-288.
The PCR primer set JW21-JW22 of Weiss et al. (19), which was reported to amplify a 139-bp fragment of the 16S rRNA gene of Helicobacter pylori, has been recently used for the...
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Resource Development and Investigation of Novel Species from Unidentified Pathogens in NCCP using MALDI-TOF MS and 16S rRNA Gene Analysis

Yu WS, Lee KM, Hwang KJ

Species identification is an important item to characterize unidentified bacterial pathogens in developing and managing bacterial resources. In this study, unidentified pathogens based on the results of an automated identification...
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Molecular Identification of Clinical Rothia Isolates from Human Patients: Proposal of a Novel Rothia Species, Rothia arfidiae sp. nov.

Ko KS, Lee MY, Park YK, Peck KR, Song JH

Four Gram-positive cocci were isolated from the cerebrospinal fluid or blood of four different patients, but they could not be identified by an automated conventional identification system, so they were...
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Taxonomic Identification of Bacillus Species Using Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry

Yu WS, Lee KM, Hwang KJ

BACKGROUND: In this study, we compared various methods of taxonomic identification of Bacillus strains: biochemical methods, 16S rRNA gene sequencing, and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)....
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Haemophilus parainfluenzae Infective Endocarditis Confirmed by 16S rRNA Sequence Analysis from Culture Negative Tissue

Park KJ, Park KS, Choi SH, Kim YJ, Ki CS, Kang IS, Lee NY

Blood culture-negative infective endocarditis (CNE) can be a diagnostic dilemma. Herein, we report a case of CNE caused by Haemophilus parainfluenzae identified only via 16S rRNA sequence analysis directly from...
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Complete Mitochondrial Genome of the Chagas Disease Vector, Triatoma rubrofasciata

Dong L, Ma X, Wang M, Zhu D, Feng Y, Zhang Y, Wang J

Triatoma rubrofasciata is a wide-spread vector of Chagas disease in Americas. In this study, we completed the mitochondrial genome sequencing of T. rubrofasciata. The total length of T. rubrofasciata mitochondrial...
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Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing Analysis

Mun S, Lee J, Lee S, Han K, Ahn TY

Sequence analysis of the 16S rRNA gene has been widely used for the classification of microorganisms. However, we have been unable to clearly identify five Flavobacterium species isolated from a...
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Analysis of Clarithromycin Resistance of Helicobacter pylori Isolated in Korea

Jeon SK, Chang MW, Kim KH, Park ID

  • KMID: 1615420
  • J Bacteriol Virol.
  • 2003 Mar;33(1):11-18.
Forty-four strains of Helicobacter pylori were isolated from Kosin Medical Center were tested of resistance to antimicrobial agents, and the mechanism of resistance to clarithromycin was investigated. We determined the...
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Campylobacter fetus Peritonitis in a Patient with Continuous Ambulatory Peritoneal Dialysis: A First Case Report in Korea

Hur K, Lee E, Kang J, Lee Y

Campylobacter fetus may cause infections such as septicemia, peritonitis, meningitis, endocarditis, septic arthritis, and cellulitis, increasing the risk of spontaneous abortion but decreasing the likelihood of gastroenteritis. We identified C....
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Isolation of Fusobacterium nucleatum from subgingival plaque in Korean

Jang HS, Kim SH, Kim HS, Kook JK, Kim MK, Yoo SY, Kim BO

The purpose of this study was to isolate and characterize the Fusobacterium nucleatum (F. nucleatum) from subgingival plaque in Korean periodontitis patients. The subgingival plaque samples of periodontitis patient were...
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Comparison of the Vitek 2, API 20A, and 16s rRNA Gene Sequencing for the Identification of Anaerobic Bacteria

Park GC, Jang SJ, Lee MJ, Kook JK, Kim MJ, Kim YS, Yang NW, Lee HS, Kang SH, Park G, Moon DS

BACKGROUND: Recently, genotypic identification of anaerobes is emerging as an alternative to the phenotypic method. In this study, we evaluated the performance of Vitek 2, API 20A and 16s rRNA...
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Complete Mitochondrial Genome of a Tongue Worm Armillifer agkistrodontis

Li J, He FN, Zheng HX, Zhang RX, Ren YJ, Hu W

Armillifer agkistrodontis (Ichthyostraca: Pantastomida) is a parasitic pathogen, only reported in China, which can cause a zoonotic disease, pentastomiasis. A complete mitochondrial (mt) genome was 16,521 bp comprising 13 protein-coding...
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