Osong Public Health Res Perspect.  2017 Feb;8(1):54-60. 10.24171/j.phrp.2017.8.1.07.

The Effect of Lactobacillus acidophilus PTCC 1643 on Cultured Intestinal Epithelial Cells Infected with Salmonella enterica serovar Enteritidis

• Moshiri M
• Dallal MM
• Rezaei F
• Douraghi M
• Sharifi L
• Noroozbabaei Z
• Gholami M
• Mirshafiey A

• KMID: 2386101
• DOI: http://doi.org/10.24171/j.phrp.2017.8.1.07

Abstract

OBJECTIVES
Gastrointestinal disorders caused by Salmonella enterica serovar Enteritidis (SesE) are a significant health problem around the globe. Probiotic bacteria have been shown to have positive effects on the immune responses. Lactobacillus acidophilus was examined for its capability to influence the innate immune response of HT29 intestinal epithelial cells towards SesE. The purpose of this work was to assess the effect of L. acidophilus PTCC 1643 on cultured intestinal epithelial cells infected with SesE.
METHODS
HT29 cells were cultured in Roswell Park Memorial Institute medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. The cells were treated with L. acidophilus PTCC 1643 after or before challenge with SesE. At 2 and 4 hours post-infection, we measured changes in the expression levels of TLR2 and TLR4 via real-time polymerase chain reaction.
RESULTS
Treatment with L. acidophilus inhibited SesE-induced increases in TLR2 and TLR4 expression in the infected HT29 cells. Moreover, the expression of TLR2 and TLR4 in cells that were pretreated with L. acidophilus and then infected with SesE was significantly higher than that in cells infected with SesE without pretreatment. Taken together, the results indicated that L. acidophilus had an anti-inflammatory effect and modulated the innate immune response to SesE by influencing TLR2 and TLR4 expression.
CONCLUSION
Our findings suggested that L. acidophilus PTCC 1643 was able to suppress inflammation caused by SesE infection in HT29 cells and reduce TLR2 and TLR4 expression. Additional in vivo and in vitro studies are required to further elucidate the mechanisms underlying this anti-inflammatory effect.

Keyword

Lactobacillus acidophilus; probiotics; Salmonella; gene expression

MeSH Terms

Bacteria
Epithelial Cells*
Gene Expression
HT29 Cells
Humans
Immunity, Innate
In Vitro Techniques
Inflammation
Lactobacillus acidophilus*
Lactobacillus*
Probiotics
Real-Time Polymerase Chain Reaction
Salmonella enterica*
Salmonella enteritidis*
Salmonella*