J Bacteriol Virol.  2013 Sep;43(3):186-194. 10.4167/jbv.2013.43.3.186.

A Virulent Salmonella enterica Serovar Enteritidis Phage SE2 with a Strong Bacteriolytic Activity of Planktonic and Biofilmed Cells

• Tiwari BR
• Kim S
• Kim J

• KMID: 2286272
• DOI: http://doi.org/10.4167/jbv.2013.43.3.186

Abstract

Salmonella enterica serovar Enteritidis is one of the major food borne pathogens. Utilizing lytic bacteriophages against this pathogen can be a new and effective approach for the prevention of food-contamination and food-borne infection. In this study, we isolated and characterized a Salmonella Enteritidis specific lytic bacteriophage (phage SE2). The bacteriolytic activity of planktonic and biofilmed cells against an antibiotic resistant strain of Salmonella Enteritidis was also evaluated. Phage SE2 revealed an efficient bacteriolytic effect with biofilm dispersing ability and could maintain its virulence even at extreme pH and temperature. It can be a potential biotherapeutic agent against Salmonella Enteritidis.

Keyword

Virulent; Salmonella Enteritidis; Bacteriophage; Drug resistance; Biofilm

MeSH Terms

Bacteriophages
Biofilms
Drug Resistance
Hydrogen-Ion Concentration
Plankton
Salmonella
Salmonella enterica
Salmonella enteritidis
Sprains and Strains

Figure

• 

  Figure 1. Adsorption rate and burst size of Phage SE2. (A) Adsorption rate of Phage SE2. (B) Burst size of Phage SE2. L, Latent time (10 minutes); b, average burst size (155 PFU/host cells at 35 minutes).

• 

  Figure 2. Ability of phage SE2 to kill bacteria and produce progenies at different temperature. (A) Ability of phage SE2 to kill bacteria at 37°C, ambient temperature and 4°C. (B) Ability of phage to produce progenies during infection at 37°C, ambient temperature (AT) and 4°C.

• 

  Figure 3. Bacteriolytic activity of the phage SE2 to biofilmed cells. (A) Biofilmed cells of Salmonella Enteritidis JB-201 were treated with tryptic soy broth (control), phage SE2 (1011 PFU/ml) or ciprofloxacin (0.5 μg/ml) for 4 hours at 37°C. Mean bacterial CFU was calculated from the triplicates. (B) Field emission scanning electron microscopic (FE-SEM) images showing biofilm dispersion activity of phage SE2. FE-SEM images showed that almost all the biofilmed cells were lysed with the dispersion of biofilm's matrix by phage SE2, very few cells, cell debris and ghost like bacteria can be seen on phage treated biofilms images (lower) compared that of phage untreated control (upper). At least three independent experiments were performed.

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