Exp Neurobiol.
2017 Jun;26(3):151-157. 10.5607/en.2017.26.3.151.
Repeated Oral Administration of Human Serum Albumin Protects from the Cerebral Ischemia in Rat Brain Following MCAO
- Affiliations
-
- 1Center for Neuroscience Imaging Research (CNIR), Institute for Basic Science (IBS), Suwon 16419, Korea. minahsuh@skku.edu
- 2Department of Biological Science, Sungkyunkwan University, Suwon 16419, Korea.
- 3Department of Chemistry and Nano Science, Ewha Womans University, Seoul 03760, Korea.
- 4Department of Biomedical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
- 5Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul 06351, Korea.
- 6Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16219, Korea.
- KMID: 2383784
- DOI: http://doi.org/10.5607/en.2017.26.3.151
Abstract
- Albumin is known to have neuroprotective effects. The protein has a long half-life circulation, and its effects can therefore persist for a long time to aid in the recovery of brain ischemia. In the present study, we investigated the neuroprotective effects of human serum albumin (HSA) on brain hemodynamics. Albumin is administrated using repeated oral gavage to the rodents. Sprague-Dawley rats underwent middle cerebral artery occlusion procedures and served as a stroke model. Afterwards, 25% human serum albumin (1.25 g/kg) or saline (5 ml/kg) was orally administrated for 2 weeks in alternating days. After 2 weeks, the rodents were assessed for levels of brain ischemia. Our testing battery consists of behavioral tests and in vivo optical imaging sessions. Modified neurological severity scores (mNSS) were obtained to assess the levels of ischemia and the effects of HSA oral administration. We found that the experimental group demonstrated larger hemodynamic responses following sensory stimulation than controls that were administered with saline. HSA administration resulted in more significant changes in cerebral blood volume following direct cortical electric stimulation. In addition, the mNSS of the treatment group was lower than the control group. In particular, brain tissue staining revealed that the infarct size was also much smaller with HSA administration. This study provides support for the efficacy of HSA, and that long-term oral administration of HSA may induce neuroprotective effects against brain ischemia.
Keyword
MeSH Terms
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Administration, Oral*
Animals
Anoxia
Behavior Rating Scale
Blood Volume
Brain Ischemia*
Brain*
Electric Stimulation
Half-Life
Hemodynamics
Humans*
Infarction, Middle Cerebral Artery
Ischemia
Neuroprotection
Neuroprotective Agents
Optical Imaging
Rats*
Rats, Sprague-Dawley
Rodentia
Serum Albumin*
Stroke
Neuroprotective Agents
Serum Albumin
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